18Oxygen incorporation into inorganic phosphate in the reaction catalyzed by N5,10-methenyltetrahydrofolate synthetase.
18Oxygen incorporation into inorganic phosphate in the reaction catalyzed by N5,10-methenyltetrahydrofolate synthetase.
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18 在 N5,10-次甲基四氢叶酸合成酶催化的反应中，氧结合到无机磷酸盐中。
DOI：
10.1016/0014-5793(95)00396-q
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发表时间：
1995
期刊：
影响因子：
3.5
通讯作者：
Himes,RH
中科院分区：
文献类型：
--
作者：
Kounga,K;VanderVelde,DG;Himes,RH
The mechanism of theN5,10-methenyltetrahydrofolate synthetase reaction was probed by determining the source of the oxygen atom introduced between the β- and γ-phosphates as ATP is converted to ADP and Pi. The reaction was performed using a mixture of [18O]- and[16O]N5-formyltetrahydrofolate in in the presence of [16O]H2O and using[16O]N5-formyltetrahydrofolate in the presence of a1:1 mixture of [18O]H2O and [16O]H2O.31P NMR spectroscopy was used to examine the products. It was found that18O from the formyl group was incorporated into Pi, and that18O was not incorporated from the solvent. The results are consistent with a mechanism involving phosphorylation of the formyl group at theN5-position, followed by displacement of the phosphate by the 10-nitrogen.
