Phytophthora infestans (Late blight) Infection Assay in a Detached Leaf of Potato
Phytophthora infestans (Late blight) Infection Assay in a Detached Leaf of Potato
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DOI：
10.21769/bioprotoc.3926
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发表时间：
2021-02-20
期刊：
影响因子：
0.8
通讯作者：
Halterman, Dennis A.
中科院分区：
文献类型：
--
作者：
Karki, Hari S.;Halterman, Dennis A.
致病疫霉是一种半生态型卵菌，主要侵染马铃薯和番茄。它侵染马铃薯和西红柿的茎、叶、块茎和果实。高通量和可重复性的感染检测是在任何作物中找到抗源的先决条件。在这个方案中，我们描述了一种分离叶试验(DLA)来进行马铃薯叶片中致病疫霉的毒力测定。马铃薯晚疫病侵染检测是一种半高通量的检测方法，可以在实验室环境中对数百种植物进行筛选。
Phytophthora infestans is a hemibiotroph oomycete that primarily infects potato and tomato. It infects stems, leaves, and tubers and fruits of potato and tomato. High throughput and reproducible infection assays are prerequisites to find sources of resistance in any crop. In this protocol, we describe a detached leaf assay (DLA) for conducting the virulence assay of P. infestans in potato leaves. A late blight infection assay using a potato detached leaf is a semi-high throughput assay in which hundreds of plants can be screened in a laboratory setting.