A MODEL FOR RNA EDITING IN KINETOPLASTID MITOCHONDRIA - GUIDE RNA MOLECULES TRANSCRIBED FROM MAXICIRCLE DNA PROVIDE THE EDITED INFORMATION
A MODEL FOR RNA EDITING IN KINETOPLASTID MITOCHONDRIA - GUIDE RNA MOLECULES TRANSCRIBED FROM MAXICIRCLE DNA PROVIDE THE EDITED INFORMATION
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DOI：
10.1016/0092-8674(90)90735-w
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发表时间：
1990-01-26
期刊：
影响因子：
64.5
通讯作者：
SIMPSON, L
中科院分区：
文献类型：
--
作者：
BLUM, B;BAKALARA, N;SIMPSON, L
一类可能参与RNA编辑的小RNA分子存在于塔伦妥利什曼原虫的细胞中。这些“向导”RNA（gRNA）分子在线粒体大环DNA的基因间区域中编码，并且含有代表编辑区域内成熟mRNA的精确互补版本的序列。此外，几种qRNA的5“部分可以与预编辑区域的3”处的mRNA形成杂交体。提出了一种模型，其中gRNA和预编辑的mRNA之间形成的部分杂交体是多个切割循环的底物，添加或缺失尿苷酸，以及重新连接，最终导致gRNA和成熟的编辑的mRNA之间的完全杂交体。
A class of small RNA molecules possibly involved in RNA editing is present in the mitochondrion of Leishmania tarentolae. These "guide" RNA (gRNA) molecules are encoded in intergenic regions of the mitochondrial maxicircle DNA and contain sequences that represent precise complementary versions of the mature mRNas within the edited regions. In addition, the 5'' portions of several qRNAs can form hybrids with mRNAs just 3'' of the preedited region. A model is presented in which a partial hybrid formed between the gRNA and preedited mRNA is substrate for multiple cycles of cleavage, addition or deletion of uridylates, and religation, eventually resulting in a complete hybrid between the gRNA and the mature edited mRNA.