Unlocking immune-mediated disease mechanisms with transcriptomics.
Unlocking immune-mediated disease mechanisms with transcriptomics.
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DOI：
10.1042/bst20200652
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发表时间：
2021-04-30
影响因子：
3.9
通讯作者：
Bosco A
中科院分区：
文献类型：
--
作者：
de Jong E;Bosco A
转录组代表了细胞中表达的RNA转录物的整个集合，反映了潜在的遗传和表观遗传景观以及环境影响，提供了在任何给定时间的功能细胞状态的全面视图。最近的技术进步现在能够在单个细胞的分辨率上研究转录组，为研究支持免疫药物疾病的细胞和分子事件提供了令人兴奋的机会。在这里，我们借鉴了最近的文献中的例子，突出了先进的生物信息学工具的应用，从散装和单细胞转录组学档案中提取机制的见解和疾病生物学。使用现有的分析技术的主要考虑因素在整个介绍。
The transcriptome represents the entire set of RNA transcripts expressed in a cell, reflecting both the underlying genetic and epigenetic landscape and environmental influences, providing a comprehensive view of functional cellular states at any given time. Recent technological advances now enable the study of the transcriptome at the resolution of individual cells, providing exciting opportunities to characterise cellular and molecular events that underpin immune-medicated diseases. Here, we draw on recent examples from the literature to highlight the application of advanced bioinformatics tools to extract mechanistic insight and disease biology from bulk and single-cell transcriptomic profiles. Key considerations for the use of available analysis techniques are presented throughout.