4D MEMRI atlas of neonatal FVB/N mouse brain development.
4D MEMRI atlas of neonatal FVB/N mouse brain development.
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DOI：
10.1016/j.neuroimage.2015.05.029
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发表时间：
2015-09
期刊：
影响因子：
5.7
通讯作者：
Turnbull DH
中科院分区：
文献类型：
--
作者：
Szulc KU;Lerch JP;Nieman BJ;Bartelle BB;Friedel M;Suero-Abreu GA;Watson C;Joyner AL;Turnbull DH
广泛使用小鼠作为研究大脑发育的模型系统，产生了对非侵入性神经成像方法的需求，这种方法可以应用于早期产后小鼠。本研究的目的是优化体内三（3D）和四维（4D）锰（Mn）增强MRI （MEMRI）方法，用于获取和分析发育中的小鼠大脑数据。自定义的，依赖于阶段的支架和自门控（运动校正）3D MRI序列的结合使得高分辨率（100 μ m各向同性），无运动伪影的大脑图像具有高水平的对比度，这是由于许多大脑区域和核的mn增强。我们获得了两组FVB/N品系小鼠的高质量纵向脑图像，每组6只小鼠，每只小鼠在奇数或偶数日交替成像（每天6张3D MEMRI图像），涵盖了出生后1至11天的发育阶段。对锰暴露、麻醉和MRI的影响进行了评估，结果显示对体重和脑容量的短暂影响很小但很显著，随着时间的推移，这些影响会恢复，与对照组相比，没有显著的形态学差异。基于变形的形态测量法（DBM）衍生的指标被用于定量分析一些大脑区域的体积、位置和信号强度的变化。小脑是一个在出生后早期经历大小和模式显著变化的大脑区域，研究人员详细分析了小脑的时空特征，从而证明了这种新的小鼠大脑发育图谱可能实现的时空特征。这些结果表明，MEMRI是定量分析小鼠大脑发育的有力工具，在小鼠神经发育疾病模型的体内表型分析中具有很大的潜力。
The widespread use of the mouse as a model system to study brain development has created the need for noninvasive neuroimaging methods that can be applied to early postnatal mice. The goal of this study was to optimize in vivo three-(3D) and four-dimensional (4D) manganese (Mn)-enhanced MRI (MEMRI) approaches for acquiring and analyzing data from the developing mouse brain. The combination of custom, stage-dependent holders and self-gated (motion-correcting) 3D MRI sequences enabled acquisition of high-resolution (100-µm isotropic), motion artifact-free brain images with a high level of contrast due to Mn-enhancement of numerous brain regions and nuclei. We acquired high-quality longitudinal brain images from two groups of FVB/N strain mice, six mice per group, each mouse imaged on alternate odd or even days (6 3D MEMRI images at each day) covering the developmental stages between postnatal days 1 to 11. The effects of Mn-exposure, anesthesia and MRI were assessed, showing small but significant transient effects on body weight and brain volume, which recovered with time and did not result in significant morphological differences when compared to controls. Metrics derived from deformation-based morphometry (DBM) were used for quantitative analysis of changes in volume, position and signal intensity of a number of brain regions. The cerebellum, a brain region undergoing significant changes in size and patterning at early postnatal stages, was analyzed in detail to demonstrate the spatiotemporal characterization made possible by this new atlas of mouse brain development. These results show that MEMRI is a powerful tool for quantitative analysis of mouse brain development, with great potential for in vivo phenotype analysis in mouse models of neurodevelopmental diseases.