Heterogeneity of cellular circadian clocks in intact plants and its correction under light-dark cycles.
Heterogeneity of cellular circadian clocks in intact plants and its correction under light-dark cycles.
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DOI：
10.1126/sciadv.1600500
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发表时间：
2016-07
期刊：
影响因子：
13.6
通讯作者：
Oyama T
中科院分区：
文献类型：
--
作者：
Muranaka T;Oyama T
植物中的细胞生物钟在光暗循环下通过纠正它们之间的异质特性来协调工作。单细胞分析的最新进展揭示了细胞过程固有的随机性和非遗传异质性。然而，我们对活的多细胞生物体中的实际细胞行为的了解仍然有限。通过使用单细胞生物发光成像技术对浮萍，浮萍，我们证明，在恒定的条件下，在完整的植物细胞工作作为个人的生物钟，振荡与自己的频率和独立响应外部刺激。定量分析揭示了细胞时钟的异质性和不稳定性以及相邻细胞之间的部分同步。此外，我们发现，在植物体内的细胞时钟下的光暗周期表现出离心相模式，其中的影响，细胞间的异质性周期长度几乎被掩盖。在恒定条件下观察到的细胞时钟的特性的固有异质性在光暗周期下被校正，以协调植物体的每日节律。这些发现为植物昼夜节律系统的时空结构研究提供了新的视角。
Cellular circadian clocks in a plant work in coordination under light-dark cycles by correcting heterogeneous traits between them. Recent advances in single-cell analysis have revealed the stochasticity and nongenetic heterogeneity inherent to cellular processes. However, our knowledge of the actual cellular behaviors in a living multicellular organism is still limited. By using a single-cell bioluminescence imaging technique on duckweed, Lemna gibba, we demonstrate that, under constant conditions, cells in the intact plant work as individual circadian clocks that oscillate with their own frequencies and respond independently to external stimuli. Quantitative analysis uncovered the heterogeneity and instability of cellular clocks and partial synchronization between neighboring cells. Furthermore, we found that cellular clocks in the plant body under light-dark cycles showed a centrifugal phase pattern in which the effect of cell-to-cell heterogeneity in period lengths was almost masked. The inherent heterogeneity in the properties of cellular clocks observed under constant conditions is corrected under light-dark cycles to coordinate the daily rhythms of the plant body. These findings provide a novel perspective of spatiotemporal architectures in the plant circadian system.