Altered Immune Regulation of Dendritic Cells and Enhanced Cytokine Production of T Cells in the Pathogenesis of Eosinophilic Chronic Rhinosinusitis
Altered Immune Regulation of Dendritic Cells and Enhanced Cytokine Production of T Cells in the Pathogenesis of Eosinophilic Chronic Rhinosinusitis
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DOI：
10.1159/000512591
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发表时间：
2021-01
影响因子：
2.8
通讯作者：
K. Kawakami;T. Miyasaka;I. Ohno;N. Ohta;Chiaki Masuda-Suzuki;Yutaka Tateda;Y. Kusano;Fumi Shoji-Fumi-Sh
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文献类型：
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作者：
K. Kawakami;T. Miyasaka;I. Ohno;N. Ohta;Chiaki Masuda-Suzuki;Yutaka Tateda;Y. Kusano;Fumi Shoji-Fumi-Sh
简介：嗜酸性粒细胞性慢性鼻窦炎（ECRS）是一种难治性慢性疾病，其特征是反复发作的鼻息肉伴严重的嗜酸性粒细胞浸润。这主要是由于增强的2型显性免疫反应，但其潜在机制仍不完全清楚。目的和方法：在本研究中，我们的目的是确定树突状细胞（DCs）和T细胞的细胞因子的个人与ECRS和年龄和性别匹配的健康对照（HC）的外周血中的特征。结果与结论：ECRS患者髓系（m）DC 1 s/DC和PD-L1+ mDC 1 s/mDC 1 s比值均高于HC患者。ECRS患者中浆细胞样（p）DC在DC中的比例以及人白细胞抗原-DR + pDC和ILT 3 + pDC在pDC中的比例低于HC。在T细胞的表征中，ECRS患者中的IL-4+ CD 4+、IFN-γ + CD 4+、IL-4+IFN-γ+ CD 4+、IL-4+ Foxp 3 + CD 4+、IFN-γ+ Foxp 3 + CD 4+、IFN-γ+IL-4− Foxp 3 − CD 4+、IL-4+ CD 8+、IL-4+IFN-γ+ CD 8+和IL-4+ Foxp 3 + CD 8 + T细胞群显著高于HC。这些结果表明，增强的mDC 1的免疫调节，减少的pDC的能力，并在外周血中的T细胞表型的比例增加可能是在ECRS发病的因素。
Introduction: Eosinophilic chronic rhinosinusitis (ECRS) is a refractory chronic disease defined by recurrent nasal polyps with severe eosinophilic infiltration. This is mainly due to enhanced type 2-dominant immune responses, but the underlying mechanism is still not fully understood. Objective and Methods: In the present study, we aimed to determine the characteristics of dendritic cells (DCs) and cytokine profiles of T cells in the peripheral blood of individuals with ECRS and age- and sex-matched healthy controls (HC). Results and Conclusion: The ratios of myeloid (m)DC1s to DCs and PD-L1+ mDC1s to mDC1s were higher in ECRS patients than in HC. The proportions of plasmacytoid (p)DCs in DCs, and human leukocyte antigen-DR+ pDCs and ILT3+ pDCs in pDCs were lower in ECRS patients than in HC. In a characterization of T cells, IL-4+CD4+, IFN-γ+CD4+, IL-4+IFN-γ+CD4+, IL-4+Foxp3+CD4+, IFN-γ+Foxp3+CD4+, IFN-γ+IL-4−Foxp3−CD4+, IL-4+CD8+, IL-4+IFN-γ+CD8+, and IL-4+Foxp3+CD8+ T-cell populations were significantly higher in ECRS patients than in HC. These results suggest that the enhanced immune regulation of mDC1, diminished capacity of pDCs, and increased proportion of the T-cell phenotypes in peripheral blood might be factors in ECRS pathogenesis.