Behavioural and pharmacological characterisation of the canopy stretched attend posture test as a model of anxiety in mice and rats
Behavioural and pharmacological characterisation of the canopy stretched attend posture test as a model of anxiety in mice and rats
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DOI：
10.1007/s002130050367
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发表时间：
1997-09-01
影响因子：
3.4
通讯作者：
Dourish, CT
中科院分区：
文献类型：
--
作者：
Grewal, SS;Shepherd, JK;Dourish, CT
行为要素，即伸展注意姿势（SAP），是啮齿类动物防御行为“风险评估”的重要组成部分。本实验范式被设计为一种在小鼠和大鼠中引发高水平 SAP 的新颖而简单的方法。 SAP 测试装置包括一个升高的黑色有机玻璃圆形平台。一个较小的透明红色有机玻璃圆形“天篷”由中心柱直接支撑在平台上方，从而将平台分为内部昏暗的覆盖区域和外部明亮的暴露区域。在该模型的大鼠和小鼠版本中，经载体处理的动物表现出对探索覆盖区域的明显偏好，并且还表现出高基线水平的 SAP，特别是在研究暴露区域时在覆盖区域边界处。在小鼠 SAP 测试中，苯二氮卓受体激动剂地西泮（0.5 mg/kg SC）和利眠宁（2 mg/kg SC）以及 5-HT1A 受体激动剂丁螺环酮（1 和 3 mg/kg SC）、伊沙匹隆（3 mg/kg SC）和 8-OH-DPAT（0.2 mg/kg SC）均显着降低 SAP 频率，且不损害运动功能活动。在大鼠 SAP 测试中，地西泮（SC 0.5 mg/kg）显着降低 SAP 发生频率，而致焦虑的 5-HT2C/1B 受体激动剂 mCPP（SC 0.25 和 0.5 mg/kg）显着增加 SAP 发生率。伊沙匹隆 (3 mg/kg SC) 诱导非特异性行为抑制。这些数据表明，“Canopy”SAP 测试是研究大鼠和小鼠风险评估行为的有用范例，并且可能提供一种敏感的新型啮齿动物焦虑模型。
The behavioural element, stretched attend posture (SAP), is an important component of the ''risk-assessment'' repertoire of defensive behaviour in rodents. The present experimental paradigm was devised as a novel and simple method of eliciting high levels of SAP in mice and rats. The SAP test apparatus comprised an elevated black Perspex circular platform. A smaller clear red Perspex circular ''Canopy'' was supported directly above the platform by a central pillar, thus dividing the platform into an inner, dimly lit covered zone and an outer, brightly lit exposed zone. In both the rat and mouse version of this model, vehicle-treated animals exhibited a marked preference for exploring the covered zone and also exhibited high baseline levels of SAP, particularly at the covered zone boundary whilst they investigated the exposed zone. In the mouse SAP test, the benzodiazepine receptor agonists, diazepam (0.5 mg/kg SC) and chlordiazepoxide (2 mg/kg SC), and the 5-HT1A receptor agonists, buspirone (1 and 3 mg/kg SC), ipsapirone (3 mg/kg SC) and 8-OH-DPAT (0.2 mg/kg SC), all significantly decreased the frequency of SAP without impairing motor activity. In the rat SAP test, diazepam (0.5 mg/kg SC) significantly decreased, whilst the anxiogenic 5-HT2C/1B receptor agonist, mCPP (0.25 and 0.5 mg/kg SC), significantly increased, the frequency of SAP. Ipsapirone (3 mg/kg SC) induced a non-specific behavioural inhibition. These data suggest that the ''Canopy'' SAP test is a useful paradigm to investigate risk assessment behaviour in both rats and mice, and may provide a sensitive novel rodent model of anxiety.