REESTABLISHING A LIFE-CYCLE OF SCHISTOSOMA-MANSONI FROM CRYOPRESERVED LARVAE
REESTABLISHING A LIFE-CYCLE OF SCHISTOSOMA-MANSONI FROM CRYOPRESERVED LARVAE
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DOI：
10.2307/3282587
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发表时间：
1989-06-01
影响因子：
1.3
通讯作者：
FILEEMPERADOR, S
中科院分区：
文献类型：
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作者：
COOPER, LA;LEWIS, FA;FILEEMPERADOR, S
为了研究从冷冻保存的曼氏血吸虫（NMRI株）幼虫重建生活史的可行性，将曼氏血吸虫成虫悬浮在冷冻保护剂1，2-乙烷中，并在液氮中冷冻保存。小鼠肌内注射液氮储存3天、3周或6个月后解冻的样品。5%的冷冻保存的幼虫和大约18%的相应的解冻对照幼虫发育成蠕虫。电阻率没有降低作为存储时间的函数。蠕虫没有表现出任何结构损伤或变化的整体大小和形态时，通过光学和透射电子显微镜检查。来自冷冻保存的幼虫的雌蠕虫具有与对照相同或略微升高的产卵量，但组织卵分布相当。随后通过Biomphalaria glabrata蜗牛和小鼠的传代显示，蜗牛的预死亡率，感染的蜗牛的百分比，每只蜗牛的尾蚴产量或尾蚴感染性没有差异。
To study the feasibility of re-establishing a life cycle of Schistosoma mansoni (NMRI strain) from cryopreserved larvae, schistosomules were suspended in the cryoprotectant 1,2-ethanediol and cryopreserved in liquid nitrogen. Mice were injected intramuscularly with samples thawed after 3 days, 3 wk, or 6 mo in liquid nitrogen storage. Two of 5% of the cryopreserved larvae and approximately 18% of corresponding unfrozen control larvae developed into adult worms. Infectivity did not decrease as a function of storage time. The adult worms showed no structural damage or changes in overall size and morphology when examined by light and transmission electron microscopy. Female worms derived from cryopreserved larvae had the same or slightly elevated egg production as controls, but tissue egg distributions were comparable. Subsequent passages through Biomphalaria glabrata snails and mice revealed no difference in snail prepatent death rate, percentage of snails infected, cercarial production per snail, or cercarial infectivity.