Genome sequence of Vibrio diabolicus and identification of the exopolysaccharide HE800 biosynthesis locus
Genome sequence of Vibrio diabolicus and identification of the exopolysaccharide HE800 biosynthesis locus
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DOI：
10.1007/s00253-014-6086-8
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发表时间：
2014-12-01
影响因子：
5
通讯作者：
Delbarre-Ladrat, Christine
中科院分区：
文献类型：
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作者：
Goudenege, David;Boursicot, Vincent;Delbarre-Ladrat, Christine
魔鬼弧菌是一种来自深海热液喷口的海洋细菌，它产生的HE 800胞外多糖具有很高的生物技术价值，尤其是对人类健康具有重要意义。其基因组进行测序和分析;使用核心基因组的系统发育分析显示，魔鬼弧菌是接近另一种深海弧菌属物种（Ex 25）的哈维进化枝和溶藻群。与来自费氏弧菌的syp簇同源的遗传位点被证明参与HE 800的生产。然而，一些遗传特性表明，syp表达的调节可能是不同的魔鬼弧菌。在基因座内存在几种类型的糖基转移酶表明在糖苷结构中产生多样性的能力，这可以赋予对环境条件的适应性。这些结果有助于更好地理解胞外多糖的生物合成，并开发新的有效的方法来生产这种分子的生物技术应用。
Vibrio diabolicus, a marine bacterium originating from deep-sea hydrothermal vents, produces the HE800 exopolysaccharide with high value for biotechnological purposes, especially for human health. Its genome was sequenced and analyzed; phylogenetic analysis using the core genome revealed V. diabolicus is close to another deep-sea Vibrio sp. (Ex25) within the Harveyi clade and Alginolyticus group. A genetic locus homologous to the syp cluster from Vibrio fischeri was demonstrated to be involved in the HE800 production. However, few genetic particularities suggest that the regulation of syp expression may be different in V. diabolicus. The presence of several types of glycosyltransferases within the locus indicates a capacity to generate diversity in the glycosidic structure, which may confer an adaptability to environmental conditions. These results contribute to better understanding exopolysaccharide biosynthesis and for developing new efficient processes to produce this molecule for biotechnological applications.