The purinergic component of human vas deferens contraction
The purinergic component of human vas deferens contraction
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DOI：
10.1016/j.fertnstert.2005.09.024
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发表时间：
2006-04-01
影响因子：
6.7
通讯作者：
Burnstock, G
中科院分区：
文献类型：
--
作者：
Banks, FCL;Knight, GE;Burnstock, G
目的：研究人类输精管中的嘌呤能信号。设计：研究阴囊输精管的收缩反应。设置：一所大学教学医院的研究部门。患者：接受输精管切除术或输精管切除术（年龄27-88岁，n = 14）。干预措施：输精管切除术或输精管切除术：主要结果测量：将输精管条悬挂在器官浴中，并进行电刺激以建立频率响应曲线。在磷酸吡哆醛-6-偶氮苯基-2 '，4'-二磺酸（PPADS，P2受体拮抗剂）、哌唑嗪（肾上腺素能α（1）拮抗剂）和河豚毒素存在下重复这些刺激。构建去甲肾上腺素和P2 X激动剂ATP和α，β-亚甲基ATP（α，β-meATP）的浓度-反应曲线。结果：在32 Hz时，PPADS使P2 X受体亚型的反应降低40%，哌唑嗪使P2 X受体亚型的反应降低80%。去甲肾上腺素引起浓度依赖性收缩（EC 50 = 11.8 μ M）。对ATP和α，β-meATP的收缩（EC 50 = 6.27 μ M）表明功能性受体是P2 X（1）和/或P2 X（3）。然而.结论：本研究证实ATP是一种与去甲肾上腺素共同参与人输精管收缩的共递质，主要通过P2 X（1）受体发挥作用。
Objective: To examine purinergic signaling in human vas deferens.Design: To study contractile responses of the scrotal vas deferens.Setting: Research department of a university teaching hospital.Patient(s): Undergoing vasectomy or orchidectomy (aged 27-88 years, n = 14). Intervention(s): Vasectomy or orchidectomy.Main Outcome Measure(s): Strips of vas deferens were suspended in an organ bath and subjected to electrical stimulation to establish frequency-response curves. These stimulations were repeated in the presence of pyridoxalphosphate-6-azophenyl-2',4'-disulfoinc acid (PPADS, P2 receptor antagonist), prazosin (adrenergic alpha(1) antagonist), and tetrodotoxin. Concentration-response curves were constructed to noradrenaline and the P2X agonists ATP and alpha,beta-methylene ATP (alpha,beta-meATP). The P2X receptor subtype distribution was assessed by immunohistochemistry using specific antibodies.Result(s): The response at 32 Hz in the presence of PPADS was reduced by 40% and in the presence of prazosin by 80%. Noradrenaline caused concentration-dependent contractions (EC50 = 11.8 mu M). Contractions to ATP and alpha,beta-meATP (EC50 = 6.27 mu M) suggested that the functional receptor was P2X(1) and/or P2X(3). However. immunohistochemistry demonstrated P2X(1), but not P2X(3), receptor immunoreactivity on the smooth muscle cells.Conclusion(s): This study demonstrated that ATP is a co-transmitter with noradrenaline in the contraction of the human vas deferens predominantly acting through the P2X(1) receptor.