NMR spectroscopy of single sub-nL ova with inductive ultra-compact single-chip probes.
NMR spectroscopy of single sub-nL ova with inductive ultra-compact single-chip probes.
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DOI：
10.1038/srep44670
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发表时间：
2017-03-20
影响因子：
4.6
通讯作者：
Boero G
中科院分区：
文献类型：
--
作者：
Grisi M;Vincent F;Volpe B;Guidetti R;Harris N;Beck A;Boero G
核磁共振 (NMR) 光谱能够对完整的生命物质进行非侵入性化学研究。然而，在微生物典型的体积尺度上使用 NMR 受到灵敏度限制的阻碍，并且迄今为止对单个完整生物体的实验仅限于体积大于 5nL 的实体。在这里，我们展示了在 0.1 和 0.5nL 的单个完整卵子上进行的核磁共振波谱实验（即比以前实现的小 10 到 50 倍），从而达到了包括人类在内的多种生物体开始生命发育的相关体积尺度。通过使用由低噪声收发器和多层 150μm 平面微线圈组成的感应式超紧凑 (1mm2) 单芯片 NMR 探头进行实验，我们证明所达到的检测限（约 5pmol 1H 核）足以检测内源性化合物。我们的研究结果表明，单芯片探针是有希望的候选者，可以在生物相关的体积尺度上进行基于核磁共振的研究和微观实体的选择。
Nuclear magnetic resonance (NMR) spectroscopy enables non-invasive chemical studies of intact living matter. However, the use of NMR at the volume scale typical of microorganisms is hindered by sensitivity limitations, and experiments on single intact organisms have so far been limited to entities having volumes larger than 5 nL. Here we show NMR spectroscopy experiments conducted on single intact ova of 0.1 and 0.5 nL (i.e. 10 to 50 times smaller than previously achieved), thereby reaching the relevant volume scale where life development begins for a broad variety of organisms, humans included. Performing experiments with inductive ultra-compact (1 mm2) single-chip NMR probes, consisting of a low noise transceiver and a multilayer 150 μm planar microcoil, we demonstrate that the achieved limit of detection (about 5 pmol of 1H nuclei) is sufficient to detect endogenous compounds. Our findings suggest that single-chip probes are promising candidates to enable NMR-based study and selection of microscopic entities at biologically relevant volume scales.