Virulent Strain of Hepatitis E Virus Genotype 3, Japan
Virulent Strain of Hepatitis E Virus Genotype 3, Japan
复制标题
DOI：
10.3201/eid1505.081100
复制
发表时间：
2009-05-01
影响因子：
11.8
通讯作者：
Mishiro, Shunji
中科院分区：
文献类型：
--
作者：
Takahashi, Kazuaki;Okamoto, Hiroaki;Mishiro, Shunji
戊型肝炎病毒（HEV）基因型3，通常会导致无症状感染，在日本，诱发8例重型肝炎。为了更好地了解与增强的毒力相关的HEV的遗传特征，我们确定了来自这8名患者和来自感染基因型3菌株swJ19的5头猪的HEV的完整或接近完整的核苷酸序列。系统发育分析表明，来自8名患者和5头猪的分离株与其他基因型3分离株分开分组，形成一个独特的簇，命名为JIO。人JIO相关病毒编码的18个氨基酸与其他HEV基因型3株不同。JIO簇中几乎所有人HEV毒株共有的一个替换位于解旋酶结构域（V239A），可能与毒力增加有关。怀疑JIO相关病毒的人畜共患来源，因为来自5头猪的分离株也具有解旋酶中的特征性V239A置换。
Hepatitis E virus (HEV) genotype 3, which usually causes asymptomatic infection in Japan, induced severe hepatitis in 8 patients. To better understand genetic features of HEV associated with increased virulence, we determined the complete or near-complete nucleotide sequences of HEV from these 8 patients and from 5 swine infected with genotype 3 strain swJ19. Phylogenetic analysis showed that the isolates from the 8 patients and the 5 swine grouped separately from the other genotype 3 isolates to create a unique cluster, designated JIO. The human JIO-related viruses encoded 18 amino acids different from those of the other HEV genotype 3 strains. One substitution common to almost all human HEV strains in the JIO cluster was located in the helicase domain (V239A) and may be associated with increased virulence. A zoonotic origin of JIO-related viruses is suspected because the isolates from the 5 swine also possessed the signature V239A substitution in helicase.