The impact of inocula carryover and inoculum dilution on the methane yields in batch methane potential tests.
The impact of inocula carryover and inoculum dilution on the methane yields in batch methane potential tests.
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DOI：
10.1016/j.biortech.2016.02.060
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发表时间：
2016-05
影响因子：
11.4
通讯作者：
Matthew Reilly;R. Dinsdale;A. Guwy
中科院分区：
文献类型：
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作者：
Matthew Reilly;R. Dinsdale;A. Guwy
批研究用于基准生物氢潜力（BHP）和生物甲烷潜力（BMP）的产量从饲料基板，碳酸盐残留物和不同的工艺配置。这项研究表明，使用纤维素的BMP产量可以通过不稀释初始污水污泥接种物而正向偏置，并且该偏置与起始接种物挥发性固体（VS）浓度无关。BHP接种物的携带也增加了BMP产量时，使用纤维素作为底物高达18.8%。此外，还观察到用去离子H2O稀释BMP接种物使来自纤维素的甲烷产率降低高达132 ± 26 N mL-CH 4g-VS-1。因此，建议甲烷分批方法中应包括接种物和标准底物对照（如本研究中所用），特别是在使用预发酵阶段（如暗发酵）时。
Batch studies are used to benchmark biohydrogen potential (BHP) and biomethane potential (BMP) yields from feed substrates, digestates residues and different process configurations. This study shows that BMP yields using cellulose can be biased positively by not diluting the initial sewage sludge inoculum and the bias is independent of starting inoculum volatile solids (VS) concentration. The carryover of BHP inoculum also increased the BMP yields when using cellulose as a substrate by up to 18.8%. Furthermore it was also observed that the dilution of BMP inoculum with deionised H2O reduced methane yields from cellulose by up to 132 ± 26 N mL-CH4g-VS−1. Therefore it is proposed that inoculum and standard substrate controls (as used in this study) should be included in methane batch methodologies, particularly when using a pre-fermentation stage such as dark fermentation.