Expression of ABO Blood Group, Hematopoietic, and Other Cell‐Specific Antigens by Cells in the Human Cornea
Expression of ABO Blood Group, Hematopoietic, and Other Cell‐Specific Antigens by Cells in the Human Cornea
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人角膜细胞表达 ABO 血型、造血和其他细胞特异性抗原
作者：
P. Treseler;G. Foulks;F. Sanfilippo
由于围绕ABO血型（ABH）抗原（可能作为移植抗原）在人角膜中的表达存在争议，我们使用高度敏感的亲和素-生物素复合物免疫过氧化物酶技术检测了这些抗原在正常角膜中的表达。ABH抗原在所有供体的角膜上皮中表达，其表达模式与其红细胞表型一致，但在基质和内皮中不存在。我们还检查了相同角膜中表达造血细胞标记的细胞，以确定哪些骨髓来源的细胞可能对角膜同种异体移植的免疫原性有贡献（如“客运白细胞”）。泛造血细胞标记物（T29/33）阳性的分散细胞遍布所有角膜，但B淋巴细胞、单核细胞或NK细胞的标记物没有一致的表达。成人角膜偶见T淋巴细胞，以角膜缘为主。最后，我们在这些角膜中寻找各种细胞特异性和结构抗原的表达，以确定角膜细胞和类似细胞群之间可能的关系。我们发现角膜内皮细胞表达一种血管内皮抗原（HuEE12），但不表达另一种（因子VIII相关抗原），这表明角膜内皮与其免疫上重要的血管对应物之间可能存在关系。各种结构抗原被发现与角膜内皮细胞、基质细胞和上皮细胞预测相关。这些发现对角膜同种异体移植反应中涉及的抗原和一些组成角膜细胞的免疫性质有了新的认识。
Because of controversy surrounding the expression of ABO blood group (ABH) antigens (which may act as transplantation antigens) in human cornea, we examined the expression of these antigens in a panel of normal corneas using a highly sensitive avidin-biotin complex immunoperoxidase technique. ABH antigens were expressed by the corneal epithelium from all donors in a pattern consistent with their red blood cell phenotype, but were absent from stroma and endothelium. We also examined the same corneas for cells expressing hematopoietic cell markers in an effort to determine which marrow-derived cells may be contributing (as “passenger leukocytes”) to the immunogenicity of corneal allografts. Scattered cells positive for a pan-hematopoietic cell marker (T29/33) were present throughout all corneas, but no consistent expression was noted of markers for B lymphocytes, monocytes, or NK cells. Adult corneas showed occasional T lymphocytes, predominantly in the limbus. Finally, we looked for the expression by these corneas of a variety of cell-specific and structural antigens, to identify possible relationships between corneal cells and analogous cell populations. We found that corneal endothelial cells expressed one vascular endothelial antigen, (HuEE12), but not another (Factor VIII related antigen), suggesting a possible relationship between corneal endothelium and its immunologically important vascular counterpart. Various structural antigens were found in predicted associations with corneal endothelial, stromal, and epithelial cells. These findings cast new light on the antigens involved in corneal allograft reactions and the immunologic nature of some constituent corneal cells.