The mouse mafB 5′-upstream fragment directs gene expression in myelomonocytic cells, differentiated macrophages and the ventral spinal cord in transgenic mice
The mouse mafB 5′-upstream fragment directs gene expression in myelomonocytic cells, differentiated macrophages and the ventral spinal cord in transgenic mice
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DOI：
10.1093/jb/mvg130
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发表时间：
2003-08-01
影响因子：
2.7
通讯作者：
Takahashi, S
中科院分区：
文献类型：
--
作者：
Hamada, M;Moriguchi, T;Takahashi, S
b-Zip 转录因子 MafB 是神经发育的重要决定因素和单核细胞分化的诱导剂。 MafB 蛋白在多种组织中表达，包括发育中的脊髓、视网膜、造血细胞的骨髓单核细胞系和腹膜巨噬细胞。然而，mafB基因表达的组织特异性调控机制及其生物学相关性尚未得到详细研究。在这里，我们首次报告使用转基因小鼠对 mafB 基因的体内调控机制和组织特异性表达进行分析。转基因包含 mafB 外显子 5' 端侧翼的 8.2-kb 序列，可特异性引导 GFP 报告基因在视网膜、造血细胞的骨髓单核细胞谱系、腹膜巨噬细胞和腹侧脊髓中表达。原位杂交分析表明，报告基因表达特异性地再现了 mafB 在视网膜和脊髓中的内源表达谱。 FACS分析显示，Gr-1、Mac-1和F4/80抗原存在于大多数来自转基因成人骨髓和脾脏的GFP阳性造血细胞上。另一方面，在检查的GFP阳性细胞中几乎不存在B220 CD4、8和Ter119细胞。这些观察结果表明，位于 mafB 上游 8.2 kb 区域的基因调控区负责指导 mafB 在视网膜、骨髓单核细胞谱系、腹膜巨噬细胞和腹侧脊髓中的表达。
The b-Zip transcription factor MafB is an essential determinant of neural development and an inducer of monocytic differentiation. The MafB protein is expressed in a variety of tissues including the developing spinal cord, retina, myelomonocytic lineages of hematopoietic cells, and peritoneal macrophages. However, the tissue-specific regulatory mechanism of mafB gene expression and its biological relevance have not been examined in detail. Here, we report, for the first time, analysis of the regulatory mechanism and tissue-specific expression of the mafB gene in vivo using transgenic mice. A transgene, containing the 8.2-kb sequence flanking the 5' end of the mafB exon, directed the expression of a GFP reporter gene specifically in the retina, myelomonocytic lineages of hematopoietic cells, peritoneal macrophages and the ventral spinal cord. In situ hybridization analysis showed that the reporter gene expression specifically recapitulates the endogenous expression profile of mafB in the retina and spinal cord. FACS analysis revealed that the Gr-1, Mac-1 and F4/80 antigens were present on most of the GFP-positive hematopoietic cells from transgenic adult bone marrow and spleen. On the other hand, B220 CD4, 8, and Ter119 cells were almost absent from among the GFP-positive cells examined. These observations suggest that gene regulatory regions located in the 8.2-kb upstream region of mafB are responsible for directing mafB expression in the retina, myelomonocytic lineages, peritoneal macrophages and the ventral spinal cord.