The pericyte as a possible osteoblast progenitor cell.
The pericyte as a possible osteoblast progenitor cell.
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DOI：
10.1097/00003086-199202000-00043
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发表时间：
1992-02
影响因子：
4.2
通讯作者：
C. Brighton;D. Lorich;R. Kupcha;T. Reilly;A. R. Jones;R. A. Woodbury
中科院分区：
文献类型：
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作者：
C. Brighton;D. Lorich;R. Kupcha;T. Reilly;A. R. Jones;R. A. Woodbury
牛毛细血管周细胞和微血管周细胞在添加10%新生小牛血清的标准组织培养液中，在不同的氧分压下单层培养10周。周细胞合成碱性磷酸酶并形成矿化的菌落。能量色散X射线光谱显示钙和磷酸盐的存在，胶原和糖胺多糖染色阳性，最重要的是，证明了骨钙素的合成。细胞增殖、羟脯氨酸合成和碱性磷酸酶合成在3%氧气中最大，骨钙素合成在3%氧气中最小。这些结果表明，毛细血管或微血管周细胞在体外表现出与体外骨细胞相似的表型表达，这些表达可能在一定程度上依赖于氧。提示毛细血管或微血管周细胞可能是成骨细胞的前体细胞。
Bovine capillary and microvessel pericytes were grown in monolayer in standard tissue culture medium supplemented with 10% newborn calf serum at various oxygen tensions for up to ten weeks. The pericytes synthesized alkaline phosphatase and formed colonies that mineralized. Energy dispersive X-ray spectrometry revealed the presence of calcium and phosphate, showed positive staining for collagen and glycosaminoglycan, and, most importantly, demonstrated the synthesis of osteocalcin. Cell proliferation, hydroxyproline production, and alkaline phosphatase synthesis were greatest in 3% oxygen, whereas osteocalcin production was least in 3% oxygen. These findings demonstrate that the capillary or microvessel pericyte exhibits phenotypic expressions in vitro that are similar to that of in vitro bone cells, and these expressions may be somewhat oxygen dependent. It is suggested from these findings that the capillary or microvessel pericyte may be an osteoblast precursor cell.