Ghrelin tissue distribution: Comparison between gene and protein expression
Ghrelin tissue distribution: Comparison between gene and protein expression
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DOI：
10.1007/bf03344083
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发表时间：
2006-02-01
影响因子：
5.4
通讯作者：
Zucchi, R
中科院分区：
文献类型：
--
作者：
Ghelardoni, S;Carnicelli, V;Zucchi, R
Ghrelin是GH促分泌素（GHS）受体的天然配体，最初从胃中分离出来，并在几种组织中检测到，但尚未对其组织分布进行系统研究。在本调查中，我们评估生长激素释放肽基因表达（RT-PCR技术）和生长激素释放肽蛋白浓度（酶免疫分析技术）从对照大鼠以及在大鼠禁食48小时获得的组织。ghrelin基因在胃、小肠、脑、小脑、垂体、心脏、胰腺、唾液腺、肾上腺、卵巢和睾丸中均有表达，其中胃中表达量最高，而肝、肺、肾和骨骼肌中表达量不明显。在胃、小肠、脑、小脑、垂体、肺、骨骼肌、胰腺、唾液腺、肾上腺、卵巢和睾丸中检测到Ghrelin蛋白，浓度范围为0.05 - 1.43 ng/mg匀浆蛋白（最高浓度出现在肺中，其次是脑）。Ghrelin在心脏、肝脏和肾脏中未检测到。因此，基因和蛋白质表达是分离的。禁食并没有产生显着的变化，生长激素释放肽基因表达，而不同组织之间的分布生长激素释放肽显着修改：蛋白质浓度增加，在大脑，小脑，肺和唾液腺，而它在胃中下降。
Ghrelin, the natural ligand of the GH secretagogue (GHS) receptor, was originally isolated from the stomach and detected in several tissues, but a systematic study of its tissue distribution has not been performed. In the present investigation, we evaluated ghrelin gene expression (by RT-PCR technique) and ghrelin protein concentration (by enzyme immunoassay technique) in tissues obtained from control rats as well as in rats subjected to 48-h fasting. The ghrelin gene was expressed in stomach, small intestine, brain, cerebellum, pituitary, heart, pancreas, salivary gland, adrenal, ovary and testis, with maximum expression occurring in the stomach, while no significant expression was detected by standard RT-PCR in liver, lung, kidney and skeletal muscle. Ghrelin protein was detected in stomach, small intestine, brain, cerebellum, pituitary, lung, skeletal muscle pancreas, salivary gland, adrenal, ovary and testis, at concentrations ranging from 0.05 to 1.43 ng/mg of homogenate protein (the highest concentration occurred in the lung, followed by the brain). Ghrelin was not detectable in the heart, liver and kidney. Therefore, gene and protein expression were dissociated. Fasting did not produce significant changes in ghrelin gene expression, while the distribution of ghrelin between different tissues was significantly modified: protein concentration increased in the brain, cerebellum, lung and salivary gland, while it decreased in the stomach.