EVIDENCE FOR GENE DUPLICATION IN COLLAGEN
EVIDENCE FOR GENE DUPLICATION IN COLLAGEN
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DOI：
10.1016/s0022-2836(76)80024-1
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发表时间：
1976-01-01
影响因子：
5.6
通讯作者：
MCLACHLAN, AD
中科院分区：
文献类型：
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作者：
MCLACHLAN, AD
将胶原蛋白α 1链的氨基酸序列与其自身在不同位移处进行比较，以寻找系统的和有意义的重复。它显示了与原纤维中相邻三螺旋分子之间的交错有关的间隔的交错，距离D为670埃。或78个（Gly-X-Y）三联体。统计学上显著的相似性仅限于序列的X系列位置，特别是疏水性氨基酸和脯氨酸，并且当比较长度为D的序列的长片段时清楚地显示。它们有两种。三个长的区域，彼此分开的155，78和233三联体，分别，显示出一致的弱同源性，这表明该序列包含3个规则的D-节段78和短的不同的节段77三联体。第二个是序列与自身相似性的不规则但非常显著的周期性变化，其在D/5，D/6，D/11和3D/5的波长处显示出强傅立叶峰。长度为D的片段的基因复制，结合序列的适应性以引导分子进入具有几种周期性包装类型的结构，产生了这些效应。
The amino acid sequence of collagen .alpha.1 chain was compared with itself at various displacements to search for systematic and significant repeats. It shows regularities at intervals related to the stagger between adjacent triple-helical molecules in the fibril, at a distance D of 670 .ANG. or 78 (Gly-X-Y) triplets. The statistically significant similarities are confined to the series X positions of the sequence, especially the hydrophobic amino acids and proline, and show clearly when long sections of sequence of length D are compared. They are of 2 kinds. Three long regions, separated from one another by 155, 78 and 233 triplets, respectively, show a consistent weak homology, suggesting that the sequence contains 3 regular D-segments of 78 and a short different segment of 77 triplets. The second is an irregular but highly significant periodic variation in the similarity of the sequence to itself, which shows strong Fourier peaks at wavelengths of D/5, D/6, D/11 and 3D/5. Gene duplication of segments of length D, combined with the adaptation of the sequence to guide the molecules into a structure with several periodic types of packing, have produced these effects.