Evolution of trans-splicing plant mitochondrial introns in pre-Permian times
Evolution of trans-splicing plant mitochondrial introns in pre-Permian times
复制标题
DOI：
10.1073/pnas.94.2.553
复制
发表时间：
1997-01-21
影响因子：
11.1
通讯作者：
Knoop, V
中科院分区：
文献类型：
--
作者：
Malek, O;Brennicke, A;Knoop, V
Trans splicing in angiosperm plant mitochondria connects exons from independent RNA molecules by means of group II intron fragments. Homologues of transsplicing introns in the angiosperm mitochondrial nad2 and nad5 genes are now identified as uninterrupted group II introns in the ferns Asplenium nidus and Marsilea drummondii. These fern introns are correctly spliced from the pre-mRNA at the sites predicted from their well-conserved secondary structures. The flanking exon sequences of the nad2 and nad5 genes in the ferns require RNA editing, including the removal of in-frame stop codons by U-to-C changes for correct expression of the genetic information. We conclude that cis-splicing introns like the ones now identified in ferns are the ancestors of trans-splicing introns in angiosperm mitochondria. Intron disruption is apparently due to a size increase of the structurally variable group II intron domain IV followed by DNA recombination in the plant mitochondrial genome.
