DISTRIBUTION OF PLECTIN, AN INTERMEDIATE FILAMENT-ASSOCIATED PROTEIN, IN THE ADULT-RAT CENTRAL-NERVOUS-SYSTEM
DISTRIBUTION OF PLECTIN, AN INTERMEDIATE FILAMENT-ASSOCIATED PROTEIN, IN THE ADULT-RAT CENTRAL-NERVOUS-SYSTEM
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DOI：
10.1002/jnr.490370411
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发表时间：
1994-03-01
影响因子：
4.2
通讯作者：
SHAW, G
中科院分区：
文献类型：
--
作者：
ERRANTE, LD;WICHE, G;SHAW, G
凝集素是一种高分子量蛋白质，最初被鉴定和表征为C6大鼠胶质瘤细胞的主要细胞骨架组分。在这里，我们证明了免疫印迹的粗中间丝（IF）蛋白制剂，plectin是一种细胞因子相关的大鼠脊髓的组成部分。然后，我们用亲和素-生物素过氧化物酶免疫细胞化学和间接免疫荧光定位在成年大鼠中枢神经系统（CNS）和检查其分布相对于IF蛋白质的凝集素。凝集素免疫反应性定位于所有室管膜细胞，包括脉络膜上皮细胞和伸长细胞、Bergmann神经胶质突起、脊髓中放射状定向的神经胶质细胞、白色物质中的星形胶质细胞、灰质中的星形胶质细胞亚群、脑干和脊髓中的运动神经元亚群以及某些内皮细胞。与神经IF蛋白的共定位研究表明，网蛋白有一个独特的分布模式，最接近的相似，但不同的波形蛋白。几个plectin阳性神经元也总是含有神经丝三联体蛋白和外周蛋白，使plectin在体外结合到三联体蛋白的能力可能反映了在体内的相互作用。在神经系统的脑室内边界以及在血脑屏障的优势是与在其他组织中的表达模式，并表明在维护这样的交界区的plectin的一般作用。(C)1994 Wiley-Liss，Inc.
Plectin is a high molecular weight protein originally identified and characterized as a major cytoskeletal component of the C6 rat glioma cell. Here we demonstrate by immunoblotting of crude intermediate filament (IF) protein preparations that plectin is a cytoskeleton-associated component of the rat spinal cord. We then used avidin-biotin peroxidase immunocytochemistry and indirect immunofluorescence to localize plectin within the adult rat central nervous system (CNS) and examine its distribution with respect to IF proteins. Plectin immunoreactivity is localized to all ependymal cells including the choroidal epithelial cells and tanycytes, Bergmann glial processes, radially oriented glial cells in the spinal cord, astrocytes in white matter, a subset of astrocytes in gray matter, a subset of motoneurons in the brainstem and spinal cord, and certain endothelial cells. Colocalization studies with neural IF proteins show that plectin has a unique distribution pattern which most closely resembles, but is distinct from, that of vimentin. The few plectin positive neurons invariably also contain the neurofilament triplet proteins and peripherin, so that the ability of plectin to bind to the triplet proteins in vitro may reflect an in vivo interaction. The predominance of plectin at the inner ventricular boundaries of the nervous system as well as at the blood-brain barrier is in line with the pattern of plectin expression in other tissues and suggests a general role for plectin in the maintenance of such junctional regions. (C) 1994 Wiley-Liss, Inc.