High-density Brassica oleracea linkage map:: identification of useful new linkages
High-density Brassica oleracea linkage map:: identification of useful new linkages
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DOI：
10.1007/s00122-007-0568-3
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发表时间：
2007-07-01
影响因子：
5.4
通讯作者：
Quiros, Carlos
中科院分区：
文献类型：
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作者：
Gao, Muqiang;Li, Genyi;Quiros, Carlos
我们构建了一个由1,257个标记组成的甘蓝高密度遗传图谱，图谱跨度为703 cM，分布在9个连锁群中，命名为LG 1-LG 9。它是在青花菜“早大”和花椰菜“安南早”的双单倍体杂交F_2分离群体中获得的。这些标记随机分布在整个图谱中，该图谱包括总共1，062个基因组SRAP标记、155个cDNA SRAP标记、26个SSR标记、3个西兰花BAC末端序列和11个已知芸苔属基因：BoGSL-ALK、BoGSL-ELONG、BoGSL-PROa、BoGSL-PROb、BoCS-裂解酶、BoGS-OH、BoCYP 79 F1、BoS-GT（芥子油苷途径）、BoDM 1（霜霉病抗性）、BoCALa、BoAP 1a（花序结构）。BoDM 1和BoGSL-ELONG在0.8cM处连锁在LG 2上，使得有可能使用硫代葡萄糖苷基因作为抗病基因的标记。通过QTL分析，我们发现了三个与花椰菜花球形成有关的片段。将该图谱与C基因组连锁群和B的染色体进行比对。oleracea和B. napus，并锚定在A. thaliana.该图谱为芸苔属分子工具增加了1,000多个新标记。
We constructed a 1,257-marker, high-density genetic map of Brassica oleracea spanning 703 cM in nine linkage groups, designated LG1-LG9. It was developed in an F2 segregating population of 143 individuals obtained by crossing double haploid plants of broccoli "Early-Big" and cauliflower "An-Nan Early". These markers are randomly distributed throughout the map, which includes a total of 1,062 genomic SRAP markers, 155 cDNA SRAP markers, 26 SSR markers, 3 broccoli BAC end sequences and 11 known Brassica genes: BoGSL-ALK, BoGSL-ELONG, BoGSL-PROa, BoGSL-PROb, BoCS-lyase, BoGS-OH, BoCYP79F1, BoS-GT (glucosinolate pathway), BoDM1 (resistance to downy mildew), BoCALa, BoAP1a (inflorescence architecture). BoDM1 and BoGSL-ELONG are linked on LG 2 at 0.8 cM, making it possible to use the glucosinolate gene as a marker for the disease resistance gene. By QTL analysis, we found three segments involved in curd formation in cauliflower. The map was aligned to the C genome linkage groups and chromosomes of B. oleracea and B. napus, and anchored to the physical map of A. thaliana. This map adds over 1,000 new markers to Brassica molecular tools.