Buckling of microtubules on elastic media via breakable bonds.
Buckling of microtubules on elastic media via breakable bonds.
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DOI：
10.1016/j.bbrc.2016.09.133
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发表时间：
2016-11
影响因子：
3.1
通讯作者：
T. Afrin;A. Kabir;K. Sada;A. Kakugo;T. Nitta
中科院分区：
文献类型：
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作者：
T. Afrin;A. Kabir;K. Sada;A. Kakugo;T. Nitta
在二维弹性介质上重建了细胞中观察到的微管屈曲，该介质由接枝在可压缩基质上的运动蛋白组成，从而能够精确控制实验条件和定量分析。然而，由于不可避免的实验困难，对观测结果的解释具有模糊性。在本研究中，我们通过计算机模拟研究了微管与弹性介质的相互作用模式在微管屈曲行为中的重要性。通过考虑微管中驱动蛋白的强制分离，我们的模拟再现了以前的实验结果，并显示了与弹性基础模型预测的偏差。另一方面，假设连接物永久地与微管结合，我们的模拟再现了弹性基础模型的预测。通过分析模拟结果，我们调查了产生这种差异的原因。这些发现表明微管与介质的相互作用模式在微管屈曲行为中的重要性。我们的发现将为活细胞中微管的屈曲提供新的见解。
Buckling of microtubules observed in cells has been reconstructed on a two-dimensional elastic medium consisting of kinesins grafted over compressible substrates, enabling precise control of experimental conditions and quantitative analysis. However, interpretations of the observations have ambiguities due to inevitable experimental difficulties. In this study, with computer simulations, we investigated importance of the mode of interaction of microtubule with elastic medium in the buckling behavior of microtubule. By taking into consideration of forced-induced detachments of kinesins from microtubules, our simulations reproduced the previous experimental results, and showed deviations from predictions of the elastic foundation model. On the other hand, with hypothetical linkers permanently bound to microtubules, our simulation reproduced the predictions of the elastic foundation model. By analyzing the results of the simulations, we investigated as to why the difference arose. These findings indicate the importance of the mode of interaction of microtubule with the medium in the buckling behavior of microtubule. Our findings would bring new insights on buckling of microtubules in living cells.