Immunization of mice with HPV vaccinia virus recombinants generates serum IgG, IgM, and mucosal IgA antibodies.
Immunization of mice with HPV vaccinia virus recombinants generates serum IgG, IgM, and mucosal IgA antibodies.
复制标题
用 HPV 痘苗病毒重组体免疫小鼠会产生血清 IgG、IgM 和粘膜 IgA 抗体。
DOI：
10.1016/s0042-6822(95)80032-8
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发表时间：
1995
期刊：
影响因子：
3.7
通讯作者：
Galloway,DA
中科院分区：
文献类型：
--
作者：
Hagensee,ME;Carter,JJ;Wipf,GC;Galloway,DA
为了评估重组牛痘病毒在发展针对HPV衣壳抗原的免疫应答中的效用，向5周龄C57 B16雌性小鼠施用由重组牛痘病毒产生的纯化的HPV 1衣壳或重组牛痘病毒本身。在第4周用任一药剂加强动物。在所有给药方案中，小鼠产生了主要针对天然L1表位的血清IgG抗体应答。最初用重组牛痘病毒注射并用纯化衣壳加强的小鼠具有更高滴度的抗体应答（P = 0.024），更多的小鼠在更大程度上应答。所有小鼠产生血清IgM应答，其先于IgG应答约2周并持续1-3周。IgM应答针对天然L1表位。虽然没有检测到血清伊加，但可以在用牛痘病毒载体免疫或加强的小鼠的阴道分泌物中检测到伊加。这些结果表明，广泛的体液免疫反应的HPV可以引起使用牛痘病毒重组。
To assess the utility of vaccinia virus recombinants in the development of an immune response against HPV capsidantigens, 5-week-old C57B16 female mice were administered either purified HPV 1 capsids produced by a vaccinia virus recombinant or the recombinant vaccinia virus itself. Animals were boosted at Week 4 with either agent. Mice developed a serum IgG antibody response in all the administration protocols that was directed mainly against native L1 epitopes. Mice injected initially with the vaccinia virus recombinant and boosted with purified capsids had a higher titer antibody response (P = 0.024) with more mice responding to a greater extent. All mice produced a serum IgM response that preceded the IgG response by approximately 2 weeks and lasted 1–3 weeks. The IgM response was directed against native Ll epitopes. Although no serum IgA was detected, IgA could be detected in vaginal secretions of mice that were immunized or boosted with the vaccinia virus vector. These results indicate that an extensive humoral immune response to HPV can be elicited using vaccinia virus recombinants.