Amperometric determination of phosphatidyl choline in serum with use of immobilized phospholipase d and choline oxidase
Amperometric determination of phosphatidyl choline in serum with use of immobilized phospholipase d and choline oxidase
复制标题
固定化磷脂酶 d 和胆碱氧化酶安培法测定血清中磷脂酰胆碱
DOI：
10.1016/s0003-2670(01)85007-8
复制
发表时间：
1979
影响因子：
6.2
通讯作者：
Shuichi Suzuki
中科院分区：
文献类型：
--
作者：
I. Karube;K. Hara;I. Satoh;Shuichi Suzuki
磷脂酶D（EC 3.1.4.4.）和胆碱氧化酶（EC 1.1.99.1.）一起固定在疏水琼脂糖凝胶上，并用于将磷脂转化为甜菜碱和过氧化氢，这是在+0.60 V vs. SCE下用安培法测量的。该传感器的响应时间为2 min，磷脂酰胆碱浓度在0-3 g l ~（-1）范围内线性关系良好。比较了不同的酶不溶性化方法。
Phospholipase D (EC 3.1.4.4.) and choline oxidase (EC 1.1.99.1.) are immobilized together on a hydrophobic agarose gel and used to convert the phospholipid to betaine and hydrogen peroxide, which is measured amperometrically at + 0.60 V vs. SCE. The response time of the sensor is 2 min, and the calibration curve for 0–3 g l-1of phosphatidyl choline is linear. Different methods of insolubilizing the enzymes are compared.