Therapy of leukemia by nonimmune syngeneic spleen cells.
Therapy of leukemia by nonimmune syngeneic spleen cells.
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通过非免疫同基因脾细胞治疗白血病。
DOI：
10.4049/jimmunol.124.5.2137
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发表时间：
1980
影响因子：
4.4
通讯作者：
A. Fefer
中科院分区：
文献类型：
--
作者：
M. Cheever;P. Greenberg;A. Fefer
在以前的研究中，携带已确诊的移植同基因白血病的小鼠可以通过过继转移免疫淋巴样细胞作为化疗的辅助手段治愈，而非免疫细胞则没有任何效果。通过使用较小的初始肿瘤接种量来提高化学免疫治疗模型的敏感性，目前的研究表明，来自正常非免疫捐赠者的细胞也可以根除肿瘤。因此，在第0天给予致死剂量(1×10(4))LSTRA的成年BALB/c小鼠在第5天接受单独的环磷酰胺(CY)或CY加非免疫细胞治疗。未经处理的小鼠在第10天全部死亡。CY单独将中位存活时间(MST)延长到第20天，但所有小鼠都死亡。CY加1×10(7)非免疫细胞治疗可将MST延长至第26天，治愈16只小鼠。正常小鼠和裸鼠脾中的效应细胞为抗Thy+C抗体的非贴壁淋巴细胞。这一结果与过继化疗免疫治疗中的效应细胞为NK细胞一致。然而，它们与经典的NK细胞不同，因为它们在体外短期内不具有直接的细胞毒性，在WINN试验中也不能中和肿瘤。
In previous studies mice bearing an established transplanted syngeneic leukemia were cured by the adoptive transfer of immune lymphoid cells inoculated as an adjunct to chemotherapy, whereas nonimmune cells had no effect. By employing a smaller initial tumor inoculum to enhance the sensitivity of the chemoimmunotherapy model, the current study demonstrated that cells from normal nonimmune donors can also eradicate tumor. Thus, adult BALB/c mice given a lethal dose (1 x 10(4)) of LSTRA on day 0 were treated on day 5 with cyclophosphamide (CY) alone or CY plus nonimmune cells. Untreated mice all died by day 10. Cy alone prolonged median survival time (MST) to day 20, but all mice died. Treatment with CY plus 1 x 10(7) nonimmune cells prolonged the MST to day 26 and cured 16 of 48 mice. The effector cells, present in the spleens of normal and nude mice, were nonadherent lymphocytes resistant to anti Thy + C. These results are consistent with this effector cell in adoptive chemoimmunotherapy being an NK cell. However, they are distinct from classical NK cells since they are not directly cytotoxic in vitro in a short term CRA and do not neutralize tumor in vivo in a Winn assay.