Effect of surfactant protein A on granular pneumocyte surfactant secretion in vitro.
Effect of surfactant protein A on granular pneumocyte surfactant secretion in vitro.
复制标题
表面活性剂蛋白 A 对体外颗粒肺细胞表面活性剂分泌的影响。
DOI：
10.1152/ajplung.00271.2002
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Fisher,AronB
中科院分区：
文献类型：
--
作者：
Bates,SandraR;Tao,Jian-Qin;Notarfrancesco,Kathleen;DeBolt,Kristine;Shuman,Henry;Fisher,AronB
Surfactant secretion by lung type II cells occurs when lamellar bodies (LBs) fuse with the plasma membrane and surfactant is released into the alveolar lumen. Surfactant protein A (SP-A) blocks secretagogue-stimulated phospholipid (PL) release, even in the presence of surfactant-like lipid. The mechanism of action is not clear. We have shown previously that an antibody to LB membranes (MAb 3C9) can be used to measure LB membrane trafficking. Although the ATP-stimulated secretion of PL was blocked by SP-A, the cell association of iodinated MAb 3C9 was not altered, indicating no effect on LB movement. FM1-43 is a hydrophobic dye used to monitor the formation of fusion pores. After secretagogue exposure, the threefold enhancement of the number of FM1-43 fluorescent LBs (per 100 cells) was not altered by the presence of SP-A. Finally, there was no evidence of a large PL pool retained on the cell surface through interaction with SP-A. Thus SP-A exposure does not affect these stages in the surfactant secretory pathway of type II cells.
