Experimental measurement of the strength of a Cα-H•••O bond in a lipid bilayer
Experimental measurement of the strength of a Cα-H•••O bond in a lipid bilayer
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DOI：
10.1021/ja049826h
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发表时间：
2004-05-05
影响因子：
15
通讯作者：
Arkin, IT
中科院分区：
文献类型：
--
作者：
Arbely, E;Arkin, IT
由于脂质双层的非极性性质，弱的 Cα−H···O H 键被认为对跨膜螺旋束（如血型糖蛋白 A (GPA)）的稳定性有显着贡献。在这里，我们首次使用含有 Gly CD2 标签的 GPA 二聚体和非二聚体变体的振动频移测量了这种键的强度。尽管由此产生的 0.88 kcal/mol 的估计粘合强度相对较弱，但几个这样的粘合可以对束的稳定做出重大贡献。
Due to the apolar nature of the lipid bilayer, the weak Cα−H···O H-bond is thought to contribute significantly toward the stability of transmembrane helical bundles such as glycophorin A (GPA). Here for the first time we measured the strength of such a bond, using vibrational frequency shifts of a dimeric and nondimeric variants of GPA containing a Gly CD2label. Although the resulting estimated bond strength of 0.88 kcal/mol is relatively weak, several such bonds could contribute significantly toward bundle stabilization.