Identification of Bacterial Strains Isolated from the Mediterranean Sea Exhibiting Different Abilities of Biofilm Formation
Identification of Bacterial Strains Isolated from the Mediterranean Sea Exhibiting Different Abilities of Biofilm Formation
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DOI：
10.1007/s00248-013-0342-9
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发表时间：
2014-07-01
影响因子：
3.6
通讯作者：
Molmeret, Maelle
中科院分区：
文献类型：
--
作者：
Brian-Jaisson, Florence;Ortalo-Magne, Annick;Molmeret, Maelle
与大西洋或太平洋等其他海洋环境相比，很少有人对地中海的海洋细菌特征进行研究。在这些环境中，从惰性表面回收的细菌的研究很少，因为已经表明附着细菌的群落结构可能与水柱中存在的浮游细菌的群落结构不同。本研究的目的是鉴定和表征从浸入地中海的惰性表面上形成的生物膜中分离出的海洋细菌，并评估它们在体外形成生物膜的能力。在这里，从法国土伦湾浸入海水的不同支撑物中分离出 13 种海洋细菌菌株。系统发育分析以及不同的生物学和物理化学特性已被研究。在回收的 13 个菌株中，鉴定出 8 个不同的属和 12 个不同的物种，其中包括 2 个新细菌物种的分离株，我们将其命名为 Persicivirga mediterranea，该属从未从地中海分离出来。希瓦氏菌属和假交替单胞菌 sp。是在我们的条件下发现的最优势的属。表型特征表明，属于Polaribacter属的一种分离株与所有其他分离株的不同之处在于其疏水性和体外形成生物膜的能力较差。识别和表征从海水（包括地中海生态系统）中分离出来的物种可能有助于了解细菌生物多样性的某些方面，并进一步研究在接近海洋环境的条件下生物膜（和生物污垢）发展的机制。
The Mediterranean Sea has rarely been investigated for the characterization of marine bacteria as compared to other marine environments such as the Atlantic or Pacific Ocean. Bacteria recovered from inert surfaces are poorly studied in these environments, when it has been shown that the community structure of attached bacteria can be dissimilar from that of planktonic bacteria present in the water column. The objectives of this study were to identify and characterize marine bacteria isolated from biofilms developed on inert surfaces immersed in the Mediterranean Sea and to evaluate their capacity to form a biofilm in vitro. Here, 13 marine bacterial strains have been isolated from different supports immersed in seawater in the Bay of Toulon (France). Phylogenetic analysis and different biological and physico-chemical properties have been investigated. Among the 13 strains recovered, 8 different genera and 12 different species were identified including 2 isolates of a novel bacterial species that we named Persicivirga mediterranea and whose genus had never been isolated from the Mediterranean Sea. Shewanella sp. and Pseudoalteromonas sp. were the most preponderant genera recovered in our conditions. The phenotypical characterization revealed that one isolate belonging to the Polaribacter genus differed from all the other ones by its hydrophobic properties and poor ability to form biofilms in vitro. Identifying and characterizing species isolated from seawater including from Mediterranean ecosystems could be helpful for example, to understand some aspects of bacterial biodiversity and to further study the mechanisms of biofilm (and biofouling) development in conditions approaching those of the marine environment.