Protective Effect of Annurca Apple Extract Against Oxidative Damage in Human Erythrocytes
Protective Effect of Annurca Apple Extract Against Oxidative Damage in Human Erythrocytes
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DOI：
10.2174/1573401311666150610210529
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发表时间：
2015-01-01
影响因子：
0.9
通讯作者：
Sammartino, Davide
中科院分区：
文献类型：
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作者：
D'Angelo, Stefania;Sammartino, Davide
苹果是世界上消费量最大的水果之一，市场上有许多不同的品种，在器官和营养特性上不同。Annurca苹果是坎帕尼亚地区的品种（意大利南部）。这种水果以脆、味道特殊和保质期长而闻名。本研究的目的是评估从阿努卡苹果（APE）中提取的多酚保护红细胞（RBC）免受氧化应激的能力。用叔丁基过氧化氢诱导人红细胞的体外氧化应激。用10 μ M APE预处理细胞提供了针对氧化损伤的显著保护，特别是降低了ROS产生（~ 35%）、脂质过骨化（~ 81%）和高铁血红蛋白形成（~ 13%）。此外，APE孵育减少红细胞形态学改变（收缩的细胞质和棘细胞的数量）所造成的t-BHP。APE的保护作用似乎与多酚类化合物的抗氧化活性有关。我们的研究结果表明，番荔枝多酚可能直接影响红细胞特性。我们的数据证实了Annurca苹果的抗氧化能力更高，因此可能代表天然抗氧化剂的可获得来源，在营养/制药领域具有潜在的应用，作为自由基参与的疾病的预防或治疗剂。
Apple is one of the most worldwide-consumed fruits and a number of cultivars, differing in organo-leptic and nutritional characteristics are available for the market. Annurca apple is a Campania region variety (Southern Italy). This fruit is known for crispness, special taste and long shelf life. The object of this study is to evaluate the capacity of polyphenols extracted from Annurca apple (APE) to protect red blood cells (RBCs) from oxidative stress. The in vitro oxidative stress on human RBCs was induced by t-butyl hydroperoxide. Pre-treatment of cells with 10 mu M APE provides a remarkable protection against oxidative damage, particularly decrease ROS production (-35%), lipid perossidation (-81%) and methemoglobin formation (-13%). Moreover, APE incubation decreased the RBC morphological alterations (shrunken cytoplasm and the number of echinocytes) caused by t-BHP. APE protective effect seems to be associated with the antioxidant activity of polyphenolic compounds. Our results indicate that Annurca polyphenols may directly influence erythrocyte properties. Our data confirm higher antioxidant power of Annurca apple, that may therefore represent accessible sources of natural antioxidants with potential application in nutritional/pharmaceutical fields, as preventive or therapeutic agents in diseases in which free radicals are implicated.