Uterine luminal epithelial alkaline phosphatase activity and pinopod development in relation to endometrial sensitivity in the rat
Uterine luminal epithelial alkaline phosphatase activity and pinopod development in relation to endometrial sensitivity in the rat
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DOI：
10.1016/s0010-7824(98)00055-9
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发表时间：
1998-07-01
期刊：
影响因子：
2.9
通讯作者：
Singh, MM
中科院分区：
文献类型：
--
作者：
Bansode, FW;Chauhan, SC;Singh, MM
大鼠子宫内膜最敏感期的特征是子宫腔上皮、腺上皮和子宫内膜间质中碱性磷酸酶活性高。子宫内膜间质中的活性随蜕膜化而增加。胞饮足发育的子宫内膜表面的第一次观察到在presentivity期间。在最敏感的那一天，它们的数量增加，在子宫的反系膜上明显多于系膜段。在避孕（p.c.）后第1天单次抗着床（1.25 mg/kg，po）剂量的centchroman对子宫内膜敏感性的抑制，虽然它在感染后第2天和第3天不影响碱性磷酸酶活性，在第4天和第5天，分别与植入前胚胎进入子宫的时间和该物种最大子宫内膜敏感性的时期相一致，导致其在子宫腔和腺上皮中的活性以及胞饮足发育的完全抑制。在这些天，除毛细血管外，整个子宫内膜基质和子宫肌层的酶活性也明显降低。相比之下，通过在子宫输卵管交界处放置结扎线来防止原生胚胎进入子宫对pinopod发育没有影响，但仅在立即植入后期间引起腔上皮和腺上皮中酶活性的显着下降。与对照组中的低立方上皮相比，centchroman处理的大鼠在最大敏感性当天的子宫腔出现高度扩张，并衬有高柱状上皮。调查结果表明：（a）子宫腔上皮碱性磷酸酶活性与子宫内膜敏感性之间的相关性;（B）Centchroman处理后腔上皮和腺上皮中酶活性的完全抑制可能与上皮细胞的渗透性特征改变有关，这与在最大敏感性当天不存在胞饮足和高度扩张的子宫腔一起，表明抑制腔液的内吞/胞饮作用、腔关闭、胚泡滋养层与腔上皮的沉积以及腺上皮的分泌活动;（c）子宫内膜表面的胞饮足发育与子宫内是否存在存活的胚泡无关;和（d）完全没有胞饮突提示子宫内膜缺乏敏感性，但它们的存在可能不一定表明大鼠的致敏子宫内膜。(C)1998年爱思唯尔科学公司All rights reserved.
The period of maximal endometrial sensitivity in the rat was characterized by high alkaline phosphatase activity in uterine luminal and glandular epithelium and endometrial stroma. The activity in endometrial stroma increased following decidualization. Pinopod development on the endometrial surface was first observed during the presensitivity period. Their number increased, apparently more so on the antimesometrial rather than the mesometrial segment of the uterus, on the day of maximal sensitivity. Inhibition in endometrial sensitivity by single anti-implantation (1.25 mg/kg, po) dose of centchroman on day 1 post-coitum (p.c.), although it did not affect alkaline phosphatase activity on days 2 and 3 p.c., caused complete inhibition in its activity in uterine luminal and glandular epithelium and pinopod development on days 4 and 5-coinciding, respectively, with time of entry of preimplantation embryos into the uterus and period of maximal endometrial sensitivity in this species. Significant decrease in enzyme activity was also evident in the entire endometrial stroma and myometrium, except blood capillaries, on these days. In comparison, prevention of entry of native embryos into the uterus by placing a ligature at the utero-tubal junction had no effect on pinopod development, but caused marked decrease in enzyme activity in luminal and glandular epithelium only during the immediate postimplantation period. The uterine lumen on the day of maximal sensitivity in centchroman-treated rats appeared highly distended and was lined with tall columnar epithelium, in comparison to low cuboidal epithelium in controls. The findings demonstrate: (a) a correlation between uterine luminal epithelial alkaline phosphatase activity and endometrial sensitivity; (b) complete inhibition in enzyme activity in luminal and glandular epithelium following centchroman treatment might be related to altered permeability characteristics of epithelial cells, which together with the absence of pinopods and highly distended uterine lumen on the day of maximal sensitivity, suggest inhibition of endocytosis/pinocytosis of luminal fluid, luminal closure, apposition of blastocyst trophoblast to luminal epithelium, and secretory activity of glandular epithelium; (c) pinopod development on the endometrial surface was independent of presence of viable blastocysts in utero; and (d) complete absence of pinopods suggests lack of endometrial sensitivity, but their presence might not necessarily indicate a sensitized endometrium in the rat. (C) 1998 Elsevier Science Inc. All rights reserved.