miR-185 enhances the inhibition of proliferation and migration induced by ionizing radiation in melanoma
miR-185 enhances the inhibition of proliferation and migration induced by ionizing radiation in melanoma
复制标题
miR-185增强对电离辐射诱导的黑色素瘤增殖和迁移的抑制
DOI：
10.3892/ol.2017.5699
复制
发表时间：
2017-04-01
期刊：
影响因子：
2.9
通讯作者：
Wang, Jufang
中科院分区：
文献类型：
--
作者：
He, Jinpeng;Tian, Ning;Wang, Jufang
黑色素瘤是一种越来越常见的侵袭性恶性肿瘤，患者存活率很低。放射治疗是黑色素瘤患者的主要选择，尤其是那些不适合手术治疗的患者；然而，由于黑色素瘤对电离辐射(IR)的相对放射抗性，其治疗效果有限。已有报道称，microRNAs(MiRNAs)在确定肿瘤的放射敏感性方面起着至关重要的作用；然而，利用miRNA对黑色素瘤进行放射增敏的研究知之甚少。本研究以C57/BL小鼠皮肤黑色素瘤细胞系B16为研究对象，研究了miRNA185(miR-185)对肾癌放射抗性的增敏作用。采用细胞增殖和划痕愈合实验检测B16细胞的增殖和迁移能力。膜联蛋白V/碘化丙啶双染色法检测IR诱导的细胞凋亡。采用体内成瘤实验检测miR-185对黑色素瘤细胞的放射增敏作用。分别用增殖标记蛋白Ki-67和苏木精-伊红染色评价细胞的增殖活性和组织学改变。本研究结果表明，miR-185在体内外均能抑制黑色素瘤细胞的增殖和迁移，增强IR诱导的细胞凋亡，抑制细胞的增殖和迁移，这为理解miRNA对黑色素瘤的放射增敏作用提供了新的思路。
Melanoma is an aggressive malignancy that is increasingly common and exhibits a poor patient survival rate. Radiotherapy is the primary option for patients with melanoma, particularly those who are not candidates for surgery; however, the therapeutic effect is limited due to the relative radioresistance of melanoma to ionizing radiation (IR). It has been reported that microRNAs (miRNAs) serve a vital role in determining the radiosensitivity of tumors; however, little is known concerning the radiosensitization of melanoma using miRNA. In the present study, the radiosensitization effect of miRNA 185 (miR-185), which has been demonstrated to reduce renal cancer radioresistance, was investigated in B16 cells, a skin melanoma cell line derived from C57/BL mice, was investigated. Cell proliferation and scratch wound healing assays were used to determine the proliferative and migratory abilities of B16 cells. Annexin V/propidium iodide double staining was used to determine the apoptosis induced by IR. A tumor formation assay was performed to determine the radiosensitization effect of miR-185 on melanoma cells in vivo. Proliferation marker protein Ki-67, and hematoxylin and eosin staining were used to assess the proliferative activity and histological changes, respectively. The results of the present study demonstrated that miR-185 suppresses cellular proliferation and migration, and enhances IR-induced apoptosis, and the inhibition of proliferation and migration, in vitro and in vivo, which provides an insight into understanding the radiosensitization of melanoma using miRNA.