Elevation of antibody against Epstein-Barr virus genes BRLF1 and BZLF1 in nasopharyngeal carcinoma
Elevation of antibody against Epstein-Barr virus genes BRLF1 and BZLF1 in nasopharyngeal carcinoma
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鼻咽癌中EB病毒基因BRLF1和BZLF1抗体的升高
DOI：
10.1007/s004320050011
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发表时间：
2000
影响因子：
3.6
通讯作者：
M. Furukawa
中科院分区：
文献类型：
--
作者：
T. Yoshizaki;H. Miwa;H. Takeshita;Hiroshi Sato;M. Furukawa
目的：eb病毒即时早期基因BZLF1和BRLF1分别编码Z蛋白和R蛋白。在鼻咽癌流行地区，抗z免疫球蛋白（Ig）抗体滴度升高是鼻咽癌（NPC）的共同特征，而R水平尚未被检测。本研究分析了日本人对Z和R的抗体滴度。方法：采用酶联免疫吸附试验（ELISA）和免疫印迹法对14例新诊断鼻咽癌患者、7例鼻咽癌完全缓解患者、15例头颈部鳞状细胞癌（HNSCC）患者和31例对照患者的血清进行检测。结果：鼻咽癌患者血清中抗-Z和抗-R igg均较缓解期、HNSCC和对照组显著升高（Z, P = 0.0016, = 0.0344, = 0.0002； R, P = 0.0015, = 0.0004, <0.0001）。通过免疫印迹分析，9例鼻咽癌患者中有9例、3例鼻咽癌缓解期患者中有1例、13例HNSCC患者中有2例检测到抗z和抗r Ig。鼻咽癌患者抗z、抗r抗体滴度比其他组高9倍。结论：ELISA法联合评价抗-Z和抗- r igg可作为筛选鼻咽癌的有效方法。
Purpose: Epstein-Barr virus immediate early genes BZLF1 and BRLF1 encode the Z and R proteins respectively. Elevation of the anti-Z immunoglobulin (Ig) antibody titer is a common feature of nasopharyngeal carcinoma (NPC) in areas where the disease endemic, whereas R levels have not been examined. This study was performed to analyze antibody titers against Z and R in Japan. Methods: Sera from 14 patients with newly diagnosed NPC, 7 with NPC in complete remission, 15 with head and neck squamous cell carcinoma (HNSCC), and 31 controls were evaluated by enzyme-linked immunosorbent assay (ELISA) and immunoblotting. Results: Both anti-Z and anti-R Ig were significantly increased in sera from patients with NPC compared with those in remission, those with HNSCC, and controls (Z, P = 0.0016, = 0.0344, = 0.0002; R, P = 0.0015, = 0.0004, <0.0001 respectively). By immunoblot analysis, anti-Z and anti-R Ig were detected in 9 of 9 cases of NPC, 1 of 3 cases NPC in remission, and 2 of 13 cases of HNSCC. Anti-Z and anti-R antibody titers were nine times higher in NPC than in other groups. None of 15 control cases showed positive reactivity against either Z or R. Conclusion: Coupled evaluation of anti-Z and anti-R Ig by ELISA might be a useful system for screening NPC.
DOI：
10.1073/pnas.93.17.9194
发表时间：
1996-08
影响因子：
11.1
作者：
S. Zalani;E. Holley-Guthrie;S. Kenney
通讯作者：
S. Zalani;E. Holley-Guthrie;S. Kenney