Functional mesenchymal stem cells remain present in bone marrow microenvironment of patients with leukemia post-allogeneic hematopoietic stem cell transplant
Functional mesenchymal stem cells remain present in bone marrow microenvironment of patients with leukemia post-allogeneic hematopoietic stem cell transplant
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异基因造血干细胞移植后，功能性间充质干细胞仍存在于白血病患者的骨髓微环境中。
DOI：
10.3109/10428194.2013.858815
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发表时间：
2014-07-01
影响因子：
2.6
通讯作者：
Wang, Heng-Xiang
中科院分区：
文献类型：
--
作者：
Ding, Li;Zhu, Heng;Wang, Heng-Xiang
间充质干细胞及其后代是骨髓微环境中重要的支持细胞。然而，骨髓间充质干细胞在造血干细胞移植(HSCT)后的功能和动力学仍不清楚。在本研究中，骨髓间充质干细胞是从15名接受造血干细胞移植的患者的76份骨髓样本中培养出来的。分别于移植前、移植后1、3、6、9个月培养骨髓成纤维细胞，计数骨髓间充质干细胞数量。长期培养造血祖细胞，观察MSCs的造血活性。并观察到MSCs对体外淋巴细胞增殖的抑制作用。结果表明，HSCT后的MSCs在体外支持造血，抑制淋巴细胞生长。此外，MSCs的数量在移植早期就减少了，并在移植后9个月恢复到基线水平。这些结果表明，功能性MSCs仍然存在于骨髓微环境中，这些发现有助于理解HSCT后骨髓微环境的重建。
Mesenchymal stem cells (MSCs) and their progenies are important supporting cells in the bone marrow (BM) microenvironment. However, the function and kinetics of MSCs post-hematopoietic stem cell transplant (HSCT) remain unknown. In the present study, MSCs were cultured from a total of 76 BM samples from 15 patients receiving HSCT. Colony-forming unit fibroblasts in BM before pre-conditioning and 1, 3, 6 and 9 months post-HSCT were cultured and counted to quantify MSCs. Hematopoiesis-supporting activity of MSCs was observed with long-term culture of hematopoietic progenitors. An inhibitory effect of MSCs on in vitro lymphocyte proliferation was also observed. Results showed that post-HSCT MSCs supported in vitro hematopoiesis and inhibited lymphocyte growth. Moreover, the quantity of MSCs was reduced at an early stage and restored to baseline level 9 months post-transplant. The results indicate that functional MSCs remain present in the BM microenvironment, and these findings shed light on the understanding of BM microenvironment reconstitution post-HSCT.