Characteristics of penile growth in pubertal rats and a non-invasive method to lengthen the penis
Characteristics of penile growth in pubertal rats and a non-invasive method to lengthen the penis
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DOI：
10.1111/andr.12845
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发表时间：
2020-11-01
期刊：
影响因子：
4.5
通讯作者：
Yuan, Jiuhong
中科院分区：
文献类型：
--
作者：
Li, Tao;Fu, Fudong;Yuan, Jiuhong
研究背景阴茎短小的男性可能存在性功能障碍和心理问题。目的探讨抑制赖氨酰氧化酶（lysyl oxidase，LOX）活性（anti-LOX）结合真空装置能否延长青春期大鼠阴茎。材料与方法购买不同年龄雄性大鼠，测量暴露阴茎长度和重量，分析阴茎海绵体蛋白表达和赖氨酰氧化酶活性。然后购买15日龄大鼠，并将其分为六组：对照组、抗赖氨酰氧化酶组、-200 mm Hg（真空装置低于-200 mm Hg值）组、-200 mm Hg +抗赖氨酰氧化酶组、-300 mm Hg组和-300 mm Hg +抗赖氨酰氧化酶组。干预7周后，测量大鼠阴茎长度并评估勃起功能。结果暴露阴茎长度和重量随年龄的增长而增加，尤其是在4 ~ 8周龄之间。阴茎海绵体中赖氨酰氧化酶活性和蛋白质表达均在2周时最高，但随着年龄的增长迅速下降，8周后缓慢下降。抗赖氨酰氧化酶使阴茎长度增加10.79%，-200 mm Hg +抗赖氨酰氧化酶使阴茎长度增加14.05%，-300 mm Hg +抗赖氨酰氧化酶使阴茎长度增加19.84%。抗赖氨酰氧化酶显着降低赖氨酰氧化酶活性，降低吡啶啉浓度;然而，它没有改变锁链素（P = 0.28），羟脯氨酸（P = 0.14），和总弹性蛋白（P = 0.06）水平。抗赖氨酰氧化酶与或不真空装置没有减少勃起功能或损害正常的显微结构的corpuscavernosum.Discussion和结论大鼠的阴茎生长高峰出现在4和8周之间。抗赖氨酰氧化酶与真空装置促进阴茎延长抑制吡啶啉生产诱导图尼卡白蛋白重塑。青春期大鼠阴茎延长作用较成年大鼠明显。没有一种手术会降低勃起功能。
Background Males with short penises may suffer from sexual dysfunction and psychological problems. However, currently, managing short penis is a huge challenge.Objectives To explore whether inhibition of lysyl oxidase (LOX) activity (anti-LOX) combined with a vacuum device could lengthen the penis of pubertal rat.Materials and Methods Male rats of different ages were purchased, their exposed penile lengths and weights were measured, and protein expression and lysyl oxidase activity in the corpus cavernosum were analyzed. Fifteen-day-old rats were then purchased and divided into six groups: control, Anti-lysyl oxidase, -200 mm Hg (vacuum device under -200 mm Hg value), -200 mm Hg + Anti-lysyl oxidase, -300 mm Hg, and -300 mm Hg + Anti-lysyl oxidase groups. After the intervention duration of 7 weeks, rats' penile length was measured and erectile function was assessed. The corpus cavernosum was harvested for histopathology and molecular assessments.Results Exposed penile length and weight significantly increased with age, especially between 4 and 8 weeks. Both the protein expression and lysyl oxidase activity in corpus cavernosum were the highest at 2 weeks; however, they quickly decreased with age and slowly declined after 8 weeks. Anti-lysyl oxidase significantly increased the penile length by 10.79% over controlled rats, -200 mm Hg + Anti-lysyl oxidase lengthened it by 14.05%, and -300 mm Hg + Anti-lysyl oxidase increased it by 19.84%. Anti-lysyl oxidase significantly reduced lysyl oxidase activity to decrease pyridinoline concentration; however, it did not change desmosine (P = .28), hydroxyproline (P = .14), and total elastin (P = .06) levels. Anti-lysyl oxidase with or without a vacuum device did not diminish erectile function or impair the normal microstructure of corpus cavernosum.Discussion and Conclusion The rats' penile growth peaks occurred between 4 and 8 weeks. Anti-lysyl oxidase with a vacuum device promoted penile lengthening by inhibiting pyridinoline production to induce tunica albuginea remodeling. The penile lengthening effect was more obvious in pubertal rats than the adult rats. None of the procedures decreased erectile function.