Injection of nerve growth factor into a low back muscle induces long-lasting latent hypersensitivity in rat dorsal horn neurons
Injection of nerve growth factor into a low back muscle induces long-lasting latent hypersensitivity in rat dorsal horn neurons
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DOI：
10.1016/j.pain.2013.05.006
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发表时间：
2013-10-01
期刊：
影响因子：
7.4
通讯作者：
Mense, Siegfried
中科院分区：
文献类型：
--
作者：
Hoheisel, Ulrich;Reuter, Ragna;Mense, Siegfried
关于腰痛患者从局部或区域性疼痛转变为广泛性疼痛的中枢机制知之甚少。该研究的目的是找出是否可以使用神经生长因子（NGF）注射诱导的肌肉输入作为动物模型，用于研究广泛的肌筋膜下腰痛的脊柱机制。在体内对大鼠背角神经元进行电生理记录，以研究间隔5天向多裂肌注射2次NGF所引起的反应性变化。已知NGF与许多疼痛性肌肉疾病密切相关。结果表明，两种NGF注射-但不是单一的-引起脊髓神经元的显着过度兴奋。第一次注射NGF后5天，神经元没有明显的致敏，但更容易通过第二次注射致敏。神经元的状态类似于伤害性启动。重要的发现是，在各种组织中具有多个感受野（RF）的神经元的比例在2次NGF注射后显著升高，并且在后肢远端出现新的RF。新的RF不位于皮肤中，而是位于深部组织（肌肉、胸腰椎筋膜）中。如果患者也出现类似的变化，这些数据可能解释了肌筋膜下腰痛的弥漫性和扩散性。(C)2013年国际疼痛研究协会。由Elsevier B出版。V.保留所有权利。
Little is known about the central mechanisms underlying the transition from local or regional to widespread pain in low back pain patients. The aim of the study was to find out if muscle input induced by injection of nerve growth factor (NGF) can be used as an animal model for studying spinal mechanisms involved in widespread myofascial low back pain. Electrophysiological recordings from rat dorsal horn neurons were made in vivo to study alterations in their responsiveness caused by 2 injections of NGF into the multifidus muscle at an interval of 5 days. NGF is known to be closely associated with many painful muscle disorders. The results demonstrate that the 2 NGF injections-but not a single one-caused a significant hyperexcitability of spinal neurons. Five days after the first NGF injection, the neurons were not significantly sensitized but were easier to sensitize by a second injection. The state of the neurons resembles nociceptive priming. Important findings were that the proportion of neurons having multiple receptive fields (RFs) in various tissues was significantly higher after 2 NGF injections, and new RFs appeared on the distal hind limb. The new RFs were located not in the skin but in deep tissues (muscles, thoracolumbar fascia). If similar changes occur in patients, the data might explain the diffuse nature and spread of myofascial low back pain. (C) 2013 International Association for the Study of Pain. Published by Elsevier B. V. All rights reserved.