PLASMA INPUT FUNCTION DETERMINATION FOR PET USING A COMMERCIAL LABORATORY ROBOT
PLASMA INPUT FUNCTION DETERMINATION FOR PET USING A COMMERCIAL LABORATORY ROBOT
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DOI：
10.1016/0969-8051(95)00042-v
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发表时间：
1995-10-01
影响因子：
3.1
通讯作者：
WOLF, AP
中科院分区：
文献类型：
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作者：
ALEXOFF, DL;SHEA, C;WOLF, AP
将商业实验室机器人系统（Zymate PyTechnology II实验室自动化系统）与标准和定制实验室设备连接，并对其进行编程，以进行人类和狒狒定量PET研究中血浆输入功能测定所需的快速放射化学测定。使用Zymark XP机械臂进行两项试验：（1）测定一系列小体积全血样本中的总血浆放射性浓度，以及（2）仅使用固相萃取法测定血浆中的原型（母体）放射性示踪剂。用于测定全血样本（0.350 mt）中总血浆放射性的稳态机器人通量为14.3个样本/h，包括自动离心、移液、称重和放射性计数。用于血浆中母体放射性示踪剂测定的机器人通量为4-6个样品/h，具体取决于放射性示踪剂。对于碳-II标记的苯扎托品，在注射后60分钟，获得了25 +/- 5.0（N = 25）、26 +/- 6.8（N = 68）、13 +/- 4.4（N = 30）、32 +/- 7.2（N = 18）、16 +/- 4.9（N = 20）作为母体放射性示踪剂的总放射性百分比，雷氯必利，哌甲酯，SR 46349 B（反式，4-[（3 Z）3-（2-二甲氨基-乙基）氧亚氨基-3（2-氟苯基）丙烯-1-基]苯酚）和可卡因分别在狒狒血浆中和84 +/- 6.4（N = 9）、18 +/- 11（N = 10）、人血浆中碳-11标记的苯托品、丙炔苯丙胺、雷氯必利和哌甲酯分别为74 +/- 5.7（N = 118）和16 +/- 3.7（N = 18）。该自动化系统已用于我们实验室常规使用的7种不同C-II标记化合物的所有血浆分析超过4年。机器人放射性示踪剂分析无人值守，包括自动清理程序，消除了所有人类与等离子体污染容器的接触。
A commercial laboratory robot system (Zymate PyTechnology II Laboratory Automation System) was interfaced to standard and custom laboratory equipment and programmed to perform rapid radiochemical assays necessary for plasma input function determination in quantitative PET studies in humans and baboons. A Zymark XP robot arm was used to carry out two assays: (1) the determination of total plasma radioactivity concentrations in a series of small-volume whole blood samples and (2) the determination of unchanged (parent) radiotracer in plasma using only solid phase extraction methods. Steady state robotic throughput for determination of total plasma radioactivity in whole blood samples (0.350 mt) is 14.3 samples/h, which includes automated centrifugation, pipetting, weighing and radioactivity counting. Robotic throughput for the assay of parent radiotracer in plasma is 4-6 samples/h depending on the radiotracer. Percents of total radioactivities present as parent radiotracers at 60 min. postinjection of 25 +/- 5.0 (N = 25), 26 +/- 6.8 (N = 68), 13 +/- 4.4 (N = 30), 32 +/- 7.2 (N = 18), 16 +/- 4.9 (N = 20), were obtained for carbon-ii labeled benztropine, raclopride, methylphenidate, SR 46349B (trans, 4-[(3Z)3-(2-dimethylamino-ethyl) oxyimino-3 (2-fluorophenyl)propen-1-yl]phenol), and cocaine respectively in baboon plasma and 84 +/- 6.4 (N = 9), 18 +/- 11 (N = 10), 74 +/- 5.7 (N = 118) and 16 +/- 3.7 (N = 18) for carbon-11 labeled benztropine, deprenyl, raclopride, and methylphenidate respectively in human plasma. The automated system has been used for more than 4 years for all plasma analyses for 7 different C-ll labeled compounds used routinely in our laboratory. The robotic radiotracer assay runs unattended and includes automated cleanup procedures that eliminates all human contact with plasma-contaminated containers.