Facile and high yielding syntheses of phosphatidylcholines and phosphatidylethanolamines containing 2H-labeled acyl chains
Facile and high yielding syntheses of phosphatidylcholines and phosphatidylethanolamines containing 2H-labeled acyl chains
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含有 2H 标记酰基链的磷脂酰胆碱和磷脂酰乙醇胺的简便高产合成
DOI：
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发表时间：
1984
期刊：
影响因子：
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通讯作者：
H. Jarrell
中科院分区：
文献类型：
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作者：
B. Perly;E. Dufourc;H. Jarrell
描述了磷脂酰胆碱和磷脂酰乙醇胺的部分化学合成的改进的可靠方法。甘油磷酰胆碱 (GPC) 作为氯化镉络合物，在二甲基甲酰胺 (DMF) 中用氘代酰酐快速酰化（3 小时），得到磷脂酰胆碱，产率 > 90%。产品不含 sn-1 和 sn-2 异构体。 2-溶血磷脂酰胆碱在氯仿中用接近化学计量的酰酐快速酰化，得到混合链磷脂酰胆碱，产率> 85%。使用磷脂酶-D，通过改进的程序从相应的磷脂酰胆碱中常规制备纯磷脂酰乙醇胺，产率 > 70%。
Improved reliable methods for the partial chemical synthesis of phosphatidylcholines and phosphatidylethanolamines are described. Glycerophosphorylcholine (GPC) as its cadmium chloride complex was acylated rapidly (3 hr) with deuterated acyl anhydrides in dimethylformamide (DMF) to give phosphatidylcholines in > 90% yields. The products were free of sn-1 and sn-2 isomers. 2-Lysophosphatidylcholines were rapidly acylated with acyl anhydrides, used in nearly stoichiometric amounts, in chloroform affording mixed-chain phosphatidylcholines in > 85% yield. Pure phosphatidylethanolamines are prepared routinely in > 70% yield by an improved procedure from the corresponding phospatidylcholines using phospholipase-D.