In vivo conjunctival reconstruction using modified PLGA grafts for decreased scar formation and contraction
In vivo conjunctival reconstruction using modified PLGA grafts for decreased scar formation and contraction
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DOI：
10.1016/s0142-9612(03)00411-3
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发表时间：
2003-12-01
期刊：
影响因子：
14
通讯作者：
Choi, JW
中科院分区：
文献类型：
--
作者：
Lee, SY;Oh, JH;Choi, JW
采用改性聚乳酸-羟基乙酸酯（PLGA） 50/50支架研究结膜的体内重建。采用NaCl溶剂铸造颗粒浸出法制备多孔PLGA基质，然后用胶原蛋白、透明质酸（HA）或/和人羊膜（AM）组分进行修饰。体外观察角膜上皮细胞和人间质成纤维细胞在支架上的生长情况。与未处理的PLGA相比，所有修饰的PLGA支架均表现出细胞粘附和增殖的增强，并且1周后增殖的细胞数量增加的顺序为PLGA < PLGA/collagen = PLGA/collagen/AM < PLGA/collagen/HA = PLGA/collagen/AM /HA，与细胞粘附在表面的顺序相同。采用双侧大白兔眼结膜创面，移植PLGA/胶原/HA支架4周，观察创面眼表组织再生情况。术后4周，所有创面均被上皮膜完全覆盖。然而，改良PLGA移植创面的收缩很小（6%），而未移植创面的收缩很大（25%）。此外，在支架内再生的胶原纤维具有随机排列的松散网络的特征，类似于天然结膜。相反，在未移植的伤口中形成了排列整齐的致密胶原纤维。我们的研究结果表明，改良的PLGA移植物作为脱细胞床可以重建受损的结膜组织，减少疤痕形成和收缩。(C) 2003 Elsevier Ltd.版权所有。
The in vivo reconstruction of conjunctiva was investigated by using modified poly(lactide-co-glycolide) (PLGA) 50/50 scaffolds. The porous PLGA matrices were prepared by a solvent-casting particulate-leaching method with NaCl, then modified with collagen, hyaluronic acid (HA) or/and human amniotic membrane (AM) component. The growth of corneal epithelial cells and human stromal fibroblasts on the scaffolds was investigated in vitro. All the modified PLGA scaffolds demonstrated enhanced cell adhesion and proliferation as compared to PLGA untreated, and the number of cells proliferated after 1 week was increased in the order of PLGA < PLGA/collagen = PLGA/collagen/AM < PLGA/collagen/HA = PLGA/coliagen/AM/HA, which was the same order as the adhesion of the cells onto the surfaces. Conjunctival wounds were created on the eyes of white rabbits bilaterally and PLGA/ collagen/HA scaffolds were grafted for 4 weeks to evaluate the regeneration of ocular surface tissue at the wounds. At postoperative 4 weeks, all the wounds were completely covered with epithelial membranes. However, the contraction was minimal (6%) in the wound grafted with the modified PLGA, while that of the ungrafted wound was substantially large (25%). In addition, the collagen fibers regenerated within the scaffolds were characterized by a random array of a loose network that resembled natural conjunctiva. On the contrary, an aligned array of dense collagen fibers formed in the ungrafted wounds. Our results indicated that the modified PLGA graft as an acellular bed might allow the reconstruction of the damaged conjunctival tissue with less scar formation and contraction. (C) 2003 Elsevier Ltd. All rights reserved.