Promotion of capacitation of guinea pig spermatozoa by the membrane mobility agent, A2C, and inhibition by the disulfide‐reducing agent, DTT
Promotion of capacitation of guinea pig spermatozoa by the membrane mobility agent, A2C, and inhibition by the disulfide‐reducing agent, DTT
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膜迁移剂 A2C 促进豚鼠精子获能，二硫键还原剂 DTT 抑制豚鼠精子获能
DOI：
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发表时间：
1982
期刊：
影响因子：
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通讯作者：
R. Yanagimachi
中科院分区：
文献类型：
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作者：
A. D. Fleming;N. Kosower;R. Yanagimachi
膜迁移剂A2C通过促进获能促进豚鼠精子顶体反应的启动。精子在无钙介质中的A2C颗粒悬浮液中孵育1小时后，在添加钙离子时发生同步、快速的顶体反应。这些顶体反应的精子能够受精，这是通过它们穿透(融合)无透明带仓鼠卵子的能力来评估的。
二硫代还原剂二硫苏糖醇(DTT)抑制A2C介导的获能。它还通过阻止精子附着在卵质膜上来阻止顶体反应的精子对无区卵子的受精。
比较了A2C对精子的作用方式和A2C诱导的体细胞融合方式。指出精子膜获能过程中的分子事件和顶体反应与其他融合事件的相似性。
DTT对获能的抑制表明膜和/或亚膜蛋白和硫醇基团在这一过程中的重要性。精子膜SH基团的氧化可能在体内获能过程中起重要作用。
The membrane mobility agent A2C accelerates the onset of the acrosome reaction of guinea pig spermatozoa by promoting capacitation. Spermatozoa incubated in a suspension of A2C particles in Ca2+-free medium for one hour undergo a synchronous, rapid acrosome reaction upon the addition of Ca2+. These acrosome-reacted spermatozoa are capable of fertilization as assessed by their ability to penetrate (fuse with) zona-free hamster eggs.
The disulfide-reducing agent, dithiothreitol (DTT) inhibits A2C-mediated capacitation. It also blocks fertilization of zone-free eggs by acrosome-reacted spermatozoa by preventing attachment of the spermatozoa to the egg plasma membrane.
The mode of A2C action on spermatozoa is compared to that of A2C-induced fusion in somatic cells. The similarity of the molecular events in the sperm membrane during capacitation and the acrosome reaction to these in other fusion events is pointed out.
Inhibition of capacitation by DTT points to the importance of membrane and/or submembrane proteins and thiol groups in this process. Oxidation of sperm membrane SH groups may play an important role in in vivo capacitation.