Modelling the effects of the pyrethroid insecticide cypermethrin on the life cycle of the soil dwelling annelid Enchytraeus crypticus, an original experimental design to calibrate a DEB-TKTD model.
Modelling the effects of the pyrethroid insecticide cypermethrin on the life cycle of the soil dwelling annelid Enchytraeus crypticus, an original experimental design to calibrate a DEB-TKTD model.
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DOI：
10.1016/j.ecoenv.2023.114499
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发表时间：
2023-01
影响因子：
6.8
通讯作者：
S. Bart;T. Jager;S. Short;A. Robinson;D. Sleep;M. Pereira;D. Spurgeon;Roman Ashauer
中科院分区：
文献类型：
--
作者：
S. Bart;T. Jager;S. Short;A. Robinson;D. Sleep;M. Pereira;D. Spurgeon;Roman Ashauer
动态能量预算理论 (DEB) 使生态毒理学家能够通过毒代动力学-毒动力学 (TK-TD) 模型的耦合来模拟化学应激源对生物体生命周期的影响。虽然 DEB-TKTD 模型在水生生物的应用方面取得了良好进展，但由于缺乏适合收集所需时间序列效应数据的专用实验设计，因此在土壤动物群中的应用很少。 Enchytraeids（环节动物门：Clitellata）是土壤生态学和生态毒理学中的模式生物。它们被认为是土壤生物活动和陆地生态系统化学胁迫的指标。尽管如此，应用 DEB-TKTD 模型来研究化学品的影响尚未在该家族上进行测试。在这里，我们评估了拟除虫菊酯杀虫剂氯氰菊酯对隐翅虫生命周期的影响。我们开发了一个原始的实验设计来收集校准该物种的 DEB-TKTD 模型所需的数据。 crypticus 呈现出缓慢的初始生长阶段，通过在 DEB 模型中添加对幼体的大小依赖性食物限制，已成功模拟该阶段。随着时间的推移，DEB-TKTD 模型模拟成功地与所有终点和治疗的数据一致。氯氰菊酯突出的生理作用模式（pMoA）是增加生长能量成本。对生存影响的阈值估计为 73.14 mg kg−1，对能量预算（即亚致死效应）影响的阈值为 19.21 mg kg−1。本研究表明 DEB-TKTD 模型可以成功应用于 E. crypticusas是一种代表性的土壤物种，可以改善陆地生态系统的生态风险评估，以及我们对非目标物种化学效应的机制理解。
The Dynamic Energy Budget theory (DEB) enables ecotoxicologists to model the effects of chemical stressors on organism life cycles through the coupling of toxicokinetic-toxicodynamic (TK-TD) models. While good progress has been made in the application of DEB-TKTD models for aquatic organisms, applications for soil fauna are scarce, due to the lack of dedicated experimental designs suitable for collecting the required time series effect data. Enchytraeids (Annelida: Clitellata) are model organisms in soil ecology and ecotoxicology. They are recognised as indicators of biological activity in soil, and chemical stress in terrestrial ecosystems. Despite this, the application of DEB-TKTD models to investigate the impact of chemicals has not yet been tested on this family. Here we assessed the impact of the pyrethroid insecticide cypermethrin on the life cycle ofEnchytraeus crypticus. We developed an original experimental design to collect the data required for the calibration of a DEB-TKTD model for this species.E. crypticuspresented a slow initial growth phase that has been successfully simulated with the addition of a size-dependent food limitation for juveniles in the DEB model. The DEB-TKTD model simulations successfully agreed with the data for all endpoints and treatments over time. The highlighted physiological mode of action (pMoA) for cypermethrin was an increase of the growth energy cost. The threshold for effects on survival was estimated at 73.14 mg kg− 1, and the threshold for effects on energy budget (i.e., sublethal effects) at 19.21 mg kg− 1. This study demonstrates that DEB-TKTD models can be successfully applied toE. crypticusas a representative soil species, and may improve the ecological risk assessment for terrestrial ecosystems, and our mechanistic understanding of chemical effects on non-target species.