Identification of a putative p53 binding sequence within the human mitochondrial genome
Identification of a putative p53 binding sequence within the human mitochondrial genome
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DOI：
10.1016/j.febslet.2004.10.099
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发表时间：
2004-12-03
期刊：
影响因子：
3.5
通讯作者：
Roemer, K
中科院分区：
文献类型：
--
作者：
Heyne, K;Mannebach, S;Roemer, K
核转录因子p53的总细胞量的一小部分似乎位于线粒体处和线粒体内。类固醇受体超家族的转录因子，如p53，缺乏一个经典的线粒体前导序列，但仍然输入到线粒体中，它们通过结合到特定的识别序列调节mtDNA的转录。在这里，我们研究了7个候选序列，从人类线粒体基因组与共识p53结合基序相似。在坐标1553处的两个不完美的半位点与核IGF-BP 3盒A结合序列同源，证明在细胞核的背景下赋予对p53和p53亲属p73 α和β的响应性。因此，线粒体p53可能直接与mtDNA结合，并可能参与线粒体转录/复制的调节。(C)2004年由Elsevier B. V.代表欧洲生物化学学会联合会出版。
A small fraction of the total cellular amount of nuclear transcription factor p53 seems to be located at and within mitochondria. Transcription factors of the steroid receptor superfamily that, like p53, lack a classical mitochondrial leader sequence are nonetheless imported into mitochondria where they regulate mtDNA transcription through binding to specific recognition sequences. Here, we examined seven candidate sequences from the human mitochondrial genome with similarity to the consensus p53 binding motif. Two imperfect half-sites at coordinate 1553 with homology to the nuclear IGF-BP3 box A binding sequence are demonstrated to confer responsivity to p53 and the p53 relatives p73alpha and beta in the context of the cell nucleus. Mitochondrial p53 may thus bind directly to mtDNA and, perhaps, be involved in the regulation of mitochondrial transcription/replication. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.