SOS INDUCTION AS AN INVIVO ASSAY OF ENZYME-DNA INTERACTIONS
SOS INDUCTION AS AN INVIVO ASSAY OF ENZYME-DNA INTERACTIONS
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DOI：
10.1016/0378-1119(91)90383-m
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发表时间：
1991-07-15
期刊：
影响因子：
3.5
通讯作者：
MODEL, P
中科院分区：
文献类型：
--
作者：
HEITMAN, J;MODEL, P
我们已经构建了方便和敏感的DNA损伤指标菌株，并描述了它们的用途。这些菌株利用了Kenyon和Walker构建的SOS：：LacZ融合。娜塔莉。阿卡德。SCI。美国77(1980)2819-2823]，并通过产生β-半乳糖苷酶对DNA损伤作出反应。它们可用于鉴定限制性内切酶系统和筛选限制性内切酶突变体。应用包括与DNA代谢有关的其他酶的研究，如DNA甲基转移酶、拓扑异构酶、重组酶以及DNA复制和修复酶。
We have constructed strains which are convenient and sensitive indicators of DNA damage and describe their use. These strains utilize an SOS::lacZ fusion constructed by Kenyon and Walker [Proc. Natl. Acad. Sci. USA 77 (1980) 2819-2823] and respond to DNA damage by producing beta-galactosidase. They can be used to characterize restriction systems and screen for restriction endonuclease mutants. Applications include the study of other enzymes involved in DNA metabolism, such as DNA methyltransferases, topoisomerases, recombinases, and DNA replication and repair enzymes.