Analysing protein-protein interactions of the Myxococcus xanthus Dif signalling pathway using the yeast two-hybrid system.
Analysing protein-protein interactions of the Myxococcus xanthus Dif signalling pathway using the yeast two-hybrid system.
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DOI：
10.1099/mic.0.27743-0
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发表时间：
2005-05
期刊：
影响因子：
1.5
通讯作者：
H. Lancero;Schryl Castaneda;N. Caberoy;Xiaoyuan Ma;A. Garza;W. Shi
中科院分区：
文献类型：
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作者：
H. Lancero;Schryl Castaneda;N. Caberoy;Xiaoyuan Ma;A. Garza;W. Shi
dif操纵子是黄色粘球菌子实体形成、原纤维（胞外多糖）产生和社会运动所必需的。dif基因座包含一个与趋化基因如mcp（difA）、cheW（difC）、cheY（difD）、cheA（difE）和cheC（difF）同源的基因簇，以及一个未知的ORF difB。本研究使用酵母双杂交分析来研究Dif蛋白之间可能的相互作用，并确定DifA、C、D和E以类似于大肠杆菌和枯草芽孢杆菌的趋化蛋白的方式相互作用。DifF与DifD相互作用，而新蛋白DifB与Dif蛋白不相互作用。此外，DifA-F蛋白被用于确定M. xanthus基因组文库作者不仅证实了已知Dif蛋白之间的特异性相互作用，而且发现了DifE和Nla 19之间以及DifB和YidC之间的两种新的相互作用，为Dif信号通路提供了一些新的信息。基于这些发现，提出了一个模型的Dif信号通路。
The dif operon is essential for fruiting body formation, fibril (exopolysaccharide) production and social motility of Myxococcus xanthus. The dif locus contains a gene cluster homologous to chemotaxis genes such as mcp (difA), cheW (difC), cheY (difD), cheA (difE) and cheC (difF), as well as an unknown ORF called difB. This study used yeast two-hybrid analysis to investigate possible interactions between Dif proteins, and determined that DifA, C, D and E interact in a similar fashion to chemotaxis proteins of Escherichia coli and Bacillus subtilis. It also showed that DifF interacted with DifD, and that the novel protein DifB did not interact with Dif proteins. Furthermore, DifA-F proteins were used to determine other possible protein-protein interactions in the M. xanthus genomic library. The authors not only confirmed the specific interactions among known Dif proteins, but also discovered two novel interactions between DifE and Nla19, and DifB and YidC, providing some new information about the Dif signalling pathway. Based on these findings, a model for the Dif signalling pathway is proposed.