Correlation between group behavior and quorum sensing in Pseudomonas aeruginosa isolated from patients with hospital-acquired pneumonia
Correlation between group behavior and quorum sensing in Pseudomonas aeruginosa isolated from patients with hospital-acquired pneumonia
复制标题
DOI：
10.3978/j.issn.2072-1439.2014.03.37
复制
发表时间：
2014-06-01
影响因子：
2.5
通讯作者：
Wan, Huan-Ying
中科院分区：
文献类型：
--
作者：
Li, Yong;Qu, Hong-Ping;Wan, Huan-Ying
背景：本研究调查了 Las 和 Rhl 群体感应 (QS) 系统的表达与从医院获得性肺炎患者中分离出的铜绿假单胞菌 (P. aeruginosa) 的群体行为（运动性、生物膜形成和绿脓素产生）之间的相关性。方法：我们分析了来自 48 名患者（30 名男性和 18 名女性；年龄）的 138 株铜绿假单胞菌分离株。 68.18 +/- 15.08 岁）。评估铜绿假单胞菌临床分离株的Las和Rhl基因表达以及细菌运动、生物膜形成和绿脓素产生。结果：铜绿假单胞菌游动、抽搐和集群运动与LasI、LasR和RhlI的表达呈正相关（P<0。05）。在所有分析时间点，发现生物膜形成与 LasI、LasR 的表达之间存在显着的正相关性 (P
Background: This study investigated the correlation between the expression of the Las and Rhl quorum-sensing (QS) systems and the communal behavior (motility, biofilm formation, and pyocyanin production) of Pseudomonas aeruginosa (P. aeruginosa) isolated from patients with hospital-acquired pneumonia.Methods: We analyzed 138 P. aeruginosa isolates from 48 patients (30 men and 18 women; age 68.18 +/- 15.08 years). P. aeruginosa clinical isolates were assessed for Las and Rhl gene expression and bacterial motility, biofilm formation, and pyocyanin production.Results: P. aeruginosa swimming, twitching, and swarming motility positively correlated with the expression of LasI, LasR, and RhlI (P0.05). At all analyzed time points, a significant positive correlation was found between biofilm formation and the expression of LasI, LasR (P