VEGF and VEGF receptor levels in retinal and brain-derived endothelial cells.
VEGF and VEGF receptor levels in retinal and brain-derived endothelial cells.
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DOI：
10.1016/s0006-291x(02)00281-4
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发表时间：
2002-05
影响因子：
3.1
通讯作者：
T. Rajah;P. Grammas
中科院分区：
文献类型：
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作者：
T. Rajah;P. Grammas
血管内皮细胞生长因子（VEGF）是一种内皮细胞特异性血管生成和通透性诱导因子，与糖尿病视网膜病变的发病机制有关。本研究的目的是比较在 5 或 30mM 葡萄糖中培养 5 天的视网膜和脑源性内皮细胞之间的 VEGF 和 VEGF 受体表达。我们的结果表明，通过流式细胞术评估，细胞表面 VEGF 受体的表达在视网膜来源的内皮细胞中较高。 RT-PCR 结果表明，视网膜和脑源性内皮细胞表达的 VEGF 水平和类型相当。暴露于 30mM 葡萄糖 5 天并没有改变 VEGF 或 VEGF 受体的水平。视网膜内皮细胞中较高水平的VEGF受体表达表明视网膜微循环可能对VEGF的作用更加敏感，这可能有助于糖尿病视网膜病变的发病机制。
Vascular endothelial cell growth factor (VEGF) is an endothelial cell-specific angiogenic and permeability-inducing factor that has been implicated in the pathogenesis of diabetic retinopathy. The objectives of this study are to compare VEGF and VEGF receptor expression between retinal and brain-derived endothelial cells cultured in 5 or 30mM glucose for 5 days. Our results show that expression of cell-surface VEGF receptors, assessed by flow cytometry, is higher in retinal-derived endothelial cells. RT-PCR results show that both retinal and brain-derived endothelial cells express comparable levels and types of VEGF. Exposure to 30mM glucose for 5 days did not alter levels of VEGF or VEGF receptors. The higher level of VEGF receptor expression in retinal endothelial cells suggests that the retinal microcirculation may be more sensitive to the effects of VEGF and this may contribute to the pathogenesis of diabetic retinopathy.