The miR156-SPL9-DFR pathway coordinates the relationship between development and abiotic stress tolerance in plants
The miR156-SPL9-DFR pathway coordinates the relationship between development and abiotic stress tolerance in plants
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DOI：
10.1111/tpj.12712
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发表时间：
2014-12-01
期刊：
影响因子：
7.2
通讯作者：
Lin, Hong-Xuan
中科院分区：
文献类型：
--
作者：
Cui, Long-Gang;Shan, Jun-Xiang;Lin, Hong-Xuan
幼体对疾病和不利条件具有较强的抵抗力。当遇到逆境时，植物的发育进程会被推迟。这种现象被认为是能量重新平衡的结果，能量重新平衡有助于植物协调发育和抗逆性之间的关系；然而，这种现象背后的分子机制仍然是个谜。在本研究中，我们发现miR156整合了环境信号以确保及时开花，从而使育种得以完成。在胁迫条件下，miR156被诱导在较长一段时间内保持幼体状态，而在有利条件下，miR156被抑制，以加速发育转换。用35S：：MIM156(通过靶标模拟)阻断拟南芥的miR156信号通路增加了植物对逆境处理的敏感性，而过表达miR156则提高了植物的逆境耐受性。事实上，这一机制在水稻中也是保守的。我们还鉴定了miR156的下游基因，即鳞状启动子结合蛋白-LIKE9(SPL9)和二氢黄素-4-还原酶(DFR)，它们通过影响花青素的代谢而参与这一过程。我们的结果揭示了植物通过miR156-Spls-DFR途径适应环境的分子机制，该途径协调发育和非生物胁迫耐受性。
Young organisms have relatively strong resistance to diseases and adverse conditions. When confronted with adversity, the process of development is delayed in plants. This phenomenon is thought to result from the rebalancing of energy, which helps plants to coordinate the relationship between development and stress tolerance; however, the molecular mechanism underlying this phenomenon remains mysterious. In this study, we found that miR156 integrates environmental signals to ensure timely flowering, thus enabling the completion of breeding. Under stress conditions, miR156 is induced to maintain the plant in the juvenile state for a relatively long period of time, whereas under favorable conditions, miR156 is suppressed to accelerate the developmental transition. Blocking the miR156 signaling pathway in Arabidopsis thaliana with 35S::MIM156 (via target mimicry) increased the sensitivity of the plant to stress treatment, whereas overexpression of miR156 increased stress tolerance. In fact, this mechanism is also conserved in Oryza sativa (rice). We also identified downstream genes of miR156, i.e. SQUAMOSA PROMOTER BINDING PROTEIN-LIKE9 (SPL9) and DIHYDROFLAVONOL-4-REDUCTASE (DFR), which take part in this process by influencing the metabolism of anthocyanin. Our results uncover a molecular mechanism for plant adaptation to the environment through the miR156-SPLs-DFR pathway, which coordinates development and abiotic stress tolerance.