Identification of a hybrid myocardial zone in the mammalian heart after birth.
Identification of a hybrid myocardial zone in the mammalian heart after birth.
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哺乳动物出生后心脏混合心肌区的鉴定
DOI：
10.1038/s41467-017-00118-1
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发表时间：
2017-07-20
影响因子：
16.6
通讯作者：
Zhou B
中科院分区：
文献类型：
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作者：
Tian X;Li Y;He L;Zhang H;Huang X;Liu Q;Pu W;Zhang L;Li Y;Zhao H;Wang Z;Zhu J;Nie Y;Hu S;Sedmera D;Zhong TP;Yu Y;Zhang L;Yan Y;Qiao Z;Wang QD;Wu SM;Pu WT;Anderson RH;Zhou B
非压实性心肌病的特点是存在广泛的小梁，这可能导致心力衰竭和恶性心律失常。小梁如何形成致密心肌尚不清楚。阐明这一过程对了解非压实病的病理生理学至关重要。在这里，我们使用遗传谱系追踪来标记Nppa+或Hey2+心肌细胞作为心室壁的小梁和致密成分。我们发现Nppa+和Hey2+心肌细胞分别来自心内膜和心外膜心室壁区。在这两个出生层之间是一个杂交区，它由来自Nppa+和Hey2+群体的细胞组成。抑制胎儿Hey2+细胞对杂交区的贡献导致出生后心脏过度小梁的持续存在。我们的研究结果表明，Hey2+胎儿致密成分的扩张及其对杂交心肌带的贡献对心室壁的正常形成至关重要。
Noncompaction cardiomyopathy is characterized by the presence of extensive trabeculations, which could lead to heart failure and malignant arrhythmias. How trabeculations resolve to form compact myocardium is poorly understood. Elucidation of this process is critical to understanding the pathophysiology of noncompaction disease. Here we use genetic lineage tracing to mark the Nppa+or Hey2+cardiomyocytes as trabecular and compact components of the ventricular wall. We find that Nppa+and Hey2+cardiomyocytes, respectively, from the endocardial and epicardial zones of the ventricular wall postnatally. Interposed between these two postnatal layers is a hybrid zone, which is composed of cells derived from both the Nppa+and Hey2+populations. Inhibition of the fetal Hey2+cell contribution to the hybrid zone results in persistence of excessive trabeculations in postnatal heart. Our findings indicate that the expansion of Hey2+fetal compact component, and its contribution to the hybrid myocardial zone, are essential for normal formation of the ventricular walls.