Response of leaf-associated bacterial communities to primary acyl-homoserine lactone in the tobacco phyllosphere
Response of leaf-associated bacterial communities to primary acyl-homoserine lactone in the tobacco phyllosphere
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烟草叶际中叶片相关细菌群落对初级酰基高丝氨酸内酯的响应
DOI：
10.1016/j.resmic.2011.11.001
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发表时间：
2012-02-01
影响因子：
2.6
通讯作者：
Zhuang, Guoqiang
中科院分区：
文献类型：
--
作者：
Lv, Di;Ma, Anzhou;Zhuang, Guoqiang
叶际被大量的附生细菌占据，这些细菌能够通过群体感应（QS）来调节它们的表型和行为。然而，参与QS的酰基高丝氨酸内酯（AHLs）对自然栖息地细菌生态的影响仍不清楚。因此，我们采用生物测定和液相色谱质谱法检测烟草叶际中的AHLs。我们的研究结果确定了几个AHLs在烟草叶际，其中大多数是短链AHLs。此外，添加外源性N-（3-oxohexanoyl）高丝氨酸内酯（3 OC 6 HSL），这是在天然存在的烟草叶圈，产生的变异性的细菌群落的组成，通过变性梯度凝胶电泳（DGGE）分析和磷脂脂肪酸（PLFA）分析确定。值得注意的是，革兰氏阳性（GP）细菌的比例增加，以11 μ M阿勒处理，但最初下降时，用10 μ M阿勒处理。这些观察结果提供了对负责AHLs的叶定殖真菌群落的组成的深入了解，特别是GP细菌，因为它们不使用AHLs作为QS的信号分子。(C)2011年巴斯德研究所。由Elsevier Masson SAS出版。All rights reserved.
The phyllosphere is inhabited by large populations of epiphytic bacteria that are able to modulate their phenotypes and behavior by quorum sensing (QS). However, the impact of acyl-homoserine lactones (AHLs) involved in QS on the ecology of bacteria in their natural habitat remains unclear. Therefore, we used a bioassay and liquid chromatography mass spectrometry to detect AHLs in the tobacco phyllosphere. Our results identified several AHLs in the tobacco phyllosphere, the majority of which were short-chain AHLs. Furthermore, the addition of an exogenous N-(3-oxohexanoyl) homoserine lactone (3OC6HSL), which is seen in the naturally occurring tobacco phyllosphere, generated variability in the composition of the bacterial community as determined by denaturing gradient gel electrophoresis (DGGE) analysis and phospholipid fatty acid (PLFA) analysis. Notably, the ratio of Gram-positive (GP) bacteria increased in response to treatment with 1 1 mu M AHL, but decreased incipiently when treated with 10 mu M AHL. These observations provide insight into the composition of the leaf-colonizing epiphyte community responsible for AHLs, particularly GP bacteria as they do not use AHLs as signaling molecules for QS. (C) 2011 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.