H1foo is indispensable for meiotic maturation of the mouse oocyte
H1foo is indispensable for meiotic maturation of the mouse oocyte
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DOI：
10.1262/jrd.19008
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发表时间：
2007-08-01
影响因子：
1.8
通讯作者：
Yoshimura, Yasunori
中科院分区：
文献类型：
--
作者：
Furuya, Masataka;Tanaka, Mamoru;Yoshimura, Yasunori
在减数分裂成熟和受精过程中，卵母细胞特异性连接蛋白H1foo定位于卵母细胞核中，扩散或结合于染色质。这种表达模式表明，H1foo在卵子发生和早期胚胎发生过程中的基因表达和染色质修饰的控制中起着关键作用。为了揭示H1foo的功能，我们显微注射针对H1foo的反义吗啉代寡核苷酸（MO）到小鼠生殖泡期卵母细胞中。反义MO组的体外成熟率明显低于对照组。注射反义MO后未能排出第一极体的卵停滞在中期I。此外，体外合成的H1foo mRNA沿着与反义MO的共注射成功地挽救了H1foo的表达并提高了体外成熟率。反义MO组孤雌生殖率与对照组无差异。这些结果表明，H1foo是必需的成熟的萌发囊泡阶段的卵母细胞。
Oocyte-specific linker histone H1foo is localized in the oocyte nucleus, either diffusely or bound to chromatin, during the processes of meiotic maturation and fertilization. This expression pattern suggests that H1foo plays a key role in the control of gene expression and chromatin modification during oogenesis and early embryogenesis. To reveal the function of H1foo, we microinjected antisense morpholino oligonucleotides (MO) against H1foo into mouse germinal-vesicle stage oocytes. The rate of in vitro maturation of the antisense MO group was significantly lower than that of the control group. Eggs that failed to extrude a first polar body following injection of antisense MO arrested at metaphase I. Additionally, co-injection of in vitro synthesized H1foo mRNA along with antisense MO successfully rescued expression of H1foo and improved the in vitro maturation rate. There was no difference in the rate of parthenogenesis between the antisense MO and control groups. These results indicate that H1foo is essential for maturation of germinal vesicle-stage oocytes.